Recombinant human EGF Protein
Recombinant Proteins
Recombinant human EGF, expressed in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture
Formulation:
Source:
Hordeum vulgare
Usage:
Our recombinant proteins are For Research Use Only. This product is not intended or approved for human, diagnostics or veterinary use
Recombinant human EGF Protein
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-200006
Formulation: Lyophilized . PBS, pH 7.2, sterile filtered.
Source: Hordeum vulgare
| Product Name | Recombinant human EGF Protein |
|---|---|
| Bioactivity | Each batch of growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Bioactivity of recombinant human EGF is assayed by measuring its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using EGF is typically< 0.1 ng/ml, corresponding to a specific activity>10 x10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines. |
| Endotoxin | Endotoxin level is less than 0.005ng per μg (0.05EU/μg) |
| Formulation | Lyophilized . PBS, pH 7.2, sterile filtered. |
| Host Species | Homo sapiens (human) |
| Description | Recombinant human EGF, expressed in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture |
| Product Background | Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture and to be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide-bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues is conserved. |
| Product Type | Recombinant Protein |
| Purity | Greater than 95% by SDS-PAGE gel analysis |
| Reconstitution | Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) |
| Source | Hordeum vulgare |
| Storage | The lyophilized human egf protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Synonym | EGF, epidermal growth factor, epidermal growth factor (beta urogastrone), pro-epidermal growth factor, beta-urogastrone, URG, HOMG4 |
| Uniprot No | P01133 |
| Usage | Our recombinant proteins are For Research Use Only. This product is not intended or approved for human, diagnostics or veterinary use |
| Last Modified | Aug 20 2021 |