Recombinant Human HMGB1 Protein (His Tag)
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-200016
Molecular Weight: The recombinant human HMGB1 consists of 226a.a and has a predicted molecular weight of 26.3 kDa. As a result of glycosylation, the apparent molecular weight of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
Formulation: Recombinant HMGB1 Protein was lyophilized from sterile PBS, pH 7.41.
Source: HEK293 Cells
|Product Name||Recombinant Human HMGB1 Protein (His Tag)|
|Molecular Weight||The recombinant human HMGB1 consists of 226a.a and has a predicted molecular weight of 26.3 kDa. As a result of glycosylation, the apparent molecular weight of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.|
|AA Sequence||Met 1-Glu 215|
|Bioactivity||1. Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 ug/ml (100 ul/well) can bind human AGER.The EC50 of human AGER is 0.27 ug/ml.2. Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is typically 20-100ug/ml.|
|Endotoxin||< 1.0 EU per μg of the protein as determined by the LAL method|
|Formulation||Recombinant HMGB1 Protein was lyophilized from sterile PBS, pH 7.41.|
|Host Species||Homo sapiens (Human)|
|Description||The human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus|
|Product Background||High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. HMG-1 belongs to the high mobility group and contains a HMG-box domain.Like the histones, HMGB1 is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in interactions with many transcription factors. It also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol.HMGB1 has been shown to play an important role in helping the RAG endonuclease form a paired complex during V(D)J recombination.|
|Product Memo||HMG-1 Protein, Human;HMG1 Protein, Human;HMG3 Protein, Human;SBP-1 Protein, Human|
|Product Type||Recombinant Protein|
|Purity||Greater than 93% as determined by SDS-PAGE|
|Stability||Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃|
|Storage||Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Synonym||HMGB1, HMG1, HMG3, SBP-1, HMG-1, high mobility group box 1|
|Usage||Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use|
|Last Modified||Aug 20 2021|