Recombinant Human IGFBP6 Protein (His Tag)
The product is for non-human research only. Not for therapeutic or veterinary use.
Catalog Number: BT-200017
Molecular Weight: The recombinant human IGFBP6 consists of 224a.a and has a predicted molecular weight of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular weight of rhIGFBP6 is approximately 36 kDa due to glycosylation.
Formulation: Recombinant IGFBP6 Protein was lyophilized from sterile PBS, pH 7.41.
Source: HEK293 Cells
|Product Name||Recombinant Human IGFBP6 Protein (His Tag)|
|Molecular Weight||The recombinant human IGFBP6 consists of 224a.a and has a predicted molecular weight of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular weight of rhIGFBP6 is approximately 36 kDa due to glycosylation.|
|AA Sequence||Met 1-Gly 240|
|Bioactivity||1. Measured by its ability to bind human IGF1 in functional ELISA.2. Measured by its ability to bind human IGF2 in functional ELISA.3. Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48 4083.). The ED50 for this effect is typically 1-5 ug/mL in the presence of 14 ng/mL human IGFII.|
|Endotoxin||< 1.0 EU per μg of the human IGFBP-6 protein as determined by the LAL method|
|Formulation||Recombinant IGFBP6 Protein was lyophilized from sterile PBS, pH 7.41.|
|Host Species||Homo sapiens (Human)|
|Description||The human IGFBP6 (P24592) (Met 1-Gly 240) was fused with a polyhistidine tag at the C-terminus|
|Product Background||Insulin-like growth factor-binding protein 6 (IGFBP-6) is a protein that in humans is encoded by the IGFBP6 gene. Insulin-like growth factor binding protein 6 (IGFBP6) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. IGFBP6 is directly downregulated by the beta-catenin/TCF complex in desmoid tumors, and imply a role for the IGF axis in the proliferation of desmoid tumors. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.|
|Product Memo||IBP6 Protein, Human; IGFBP-6 Protein, Human|
|Product Type||Recombinant Protein|
|Purity||Greater than 96% as determined by SDS-PAGE|
|Stability||Recombinant Human IGFBP6 Proteins are stable for up to 1 year from date of receipt at -70℃|
|Storage||Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Synonym||IGFBP6, IBP6, insulin like growth factor binding protein 6|
|Usage||Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use|
|Last Modified||Aug 20 2021|