Recombinant WISP1 Protein, Human (His Tag) Recombinant Proteins The human WISP1 (NP_003873.1) (Met 1-Asn 367) was expressed with a C-terminal polyhistidine tag The secreted recombinant human WISP1 consisting of 356a.a has a calculated molecular weight of 39 kDa. As a result of glycosylation, It migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
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Formulation: The secreted recombinant human WISP1 consisting of 356a.a has a calculated molecular weight of 39 kDa. As a result of glycosylation, It migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant WISP1 Protein, Human (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog No: bt-200069

Molecular Weight: The secreted recombinant human WISP1 consisting of 356a.a has a calculated molecular weight of 39 kDa. As a result of glycosylation, It migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.

Formulation: Recombinant WISP1 Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant WISP1 Protein, Human (His Tag)
Molecular Weight The secreted recombinant human WISP1 consisting of 356a.a has a calculated molecular weight of 39 kDa. As a result of glycosylation, It migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
AA Sequence Met 1-Asn 367
Bioactivity Measured in a cell proliferation assay using NRK-49F rat fibroblasts (Xu, L. et al., 2000, Gene and Dev. 14 585.). The ED50 for this effect is typically 10-25 ug/ml.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant WISP1 Protein was lyophilized from sterile PBS, pH 7.41
Host Species Homo sapiens (Human)
Description The human WISP1 (NP_003873.1) (Met 1-Asn 367) was expressed with a C-terminal polyhistidine tag
Product Background CCN4/Wnt-induced secreted protein 1 (WISP1) is a secreted, cysteine-rich, heparin-binding glycoprotein, belonging to the CCN (CTGF/CYR61/NOV) family of growth factors, and is involved in diverse biological functions such as cell growth, adhesion, migration, angiogenesis, tissue repair, and regulation of extracellular matrix. Members of the CCN family demonstrate high structural homology sharing four conserved cysteine-rich modular domains: a IGFBP (insulin-like growthfactor-binding) domain, a von Willebrand type C domain, a thrombospondin domain and a C-terminal cysteine -knot domain. WISP1 is a putative downstream effector of the Wnt/Frizzled pathway that mediates diversedevelopmental processes, was identified as an oncogene regulated by the Wnt-1-beta-catenin pathway. Thus may contributes to Wnt-1-mediated tumorigenesis and malignance. Expression of WISP1 in some cells results in transformation and tumorigenesis. WISP1 acts to block cell death at a late stage in the p53-mediated apoptosis pathway. It was reported that WISP1 interacts with sulfated glycoconjugates, decorin and biglycan in the ECM of connective tissue, and possibly prevents their inhibitory activity in tumor cell proliferation.
Product Memo CCN4 Protein, Human;WISP1c Protein, Human;WISP1i Protein, Human;WISP1tc Protein, Human
Product Type Recombinant Protein
Purity Greater than 95% as determined by SDS-PAGE
Source HEK293 Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Thr 23
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Mar 08 2019