Recombinant PVRL1 Protein, Rat (His Tag) Recombinant Proteins The rat PVRL1 (XP_236210.3) (Met1-Ala354) was expressed, fused with a polyhistidine tag at the C-terminus The recombinant rat PVRL1 comprises 335a.a and predicts a molecular weight of 37.6 kDa. The apparent molecular weight of the recombinant protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
$ $99 In stock
Formulation: The recombinant rat PVRL1 comprises 335a.a and predicts a molecular weight of 37.6 kDa. The apparent molecular weight of the recombinant protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant PVRL1 Protein, Rat (His Tag)

Formulation: Recombinant PVRL1 Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant PVRL1 Protein, Rat (His Tag)
Product Type Recombinant Protein
Source HEK293 Cells
Formulation Recombinant PVRL1 Protein was lyophilized from sterile PBS, pH 7.41
Molecular Weight The recombinant rat PVRL1 comprises 335a.a and predicts a molecular weight of 37.6 kDa. The apparent molecular weight of the recombinant protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 97% as determined by SDS-PAGE
Description The rat PVRL1 (XP_236210.3) (Met1-Ala354) was expressed, fused with a polyhistidine tag at the C-terminus
Host Species Rat
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met1-Ala354
Product Background Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
Terminal Gln 31
Bioactivity Please contact us for detailed information