Recombinant Osteopontin Protein, Human (His Tag) Others The pro form of human SPP1 (NP_001035147.1) (Met 1-Asn 314) was fused with a polyhistidine tag at the C-terminus The recombinant human SPP1 consists of 309a.a after removal of the signal peptide and has a calculated molecular weight of 35 kDa. The apparent molecular weight of rh SPP1 is approximately 60-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Formulation: The recombinant human SPP1 consists of 309a.a after removal of the signal peptide and has a calculated molecular weight of 35 kDa. The apparent molecular weight of rh SPP1 is approximately 60-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Recombinant Osteopontin Protein, Human (His Tag) -

Recombinant Osteopontin Protein, Human (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-201313

Molecular Weight: The recombinant human SPP1 consists of 309a.a after removal of the signal peptide and has a calculated molecular weight of 35 kDa. The apparent molecular weight of rh SPP1 is approximately 60-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Formulation: Recombinant Osteopontin Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant Osteopontin Protein, Human (His Tag)
Molecular Weight The recombinant human SPP1 consists of 309a.a after removal of the signal peptide and has a calculated molecular weight of 35 kDa. The apparent molecular weight of rh SPP1 is approximately 60-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
AA Sequence Met 1-Asn 314
Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.When cells are added to coated plates(2ug/mL, 100uL/well), approximately 60% will adhere after 1 hour of incubation at 37℃.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant Osteopontin Protein was lyophilized from sterile PBS, pH 7.41
Host Species Homo sapiens (Human)
Description The pro form of human SPP1 (NP_001035147.1) (Met 1-Asn 314) was fused with a polyhistidine tag at the C-terminus
Product Background Osteopontin, also known as Secreted phosphoprotein 1, Bone sialoprotein 1, BSP-1, OPN, and SPP1, is a member of the osteopontin family and a SIBLING glycoprotein. Osteopontin has been classified as T-helper 1 cytokine and thus believed to exacerbate inflammation in several chronic inflammatory diseases, including atherosclerosis. Besides proinflammatory functions, physiologically Osteopontin is a potent inhibitor of mineralization, it prevents ectopic calcium deposits and is a potent inducible inhibitor of vascular calcification. Osteopontin is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Osteopontin recruitment functions of inflammatory cells are thought to be mediated through its adhesive domains, especially the arginine-glycine-aspartate (RGD) sequence that interacts with several integrin heterodimers. Osteopontin has emerged as a potential biomarker and mediator in cardiovascular disease. In the context of atherosclerosis, OPN is generally regarded as a proinflammatory and proatherogenic molecule. However, the role of OPN in vascular calcification (VC), which is closely related to chronic and active inflammation, is that of a negative regulator because it is an inhibitor of calcification and an active inducer of decalcification. Extensive research has demonstrated the pivotal participation of Osteopontin in the regulation of cell signaling which controls neoplastic and malignant transformation. The elevated expression of Osteopontin has been observed in a variety of cancers. It has been linked with tumor metastasis and signifies a poor prognosis for the patient.
Product Memo BNSP Protein, Human;BSPI Protein, Human;ETA-1 Protein, Human;OPN Protein, Human
Product Type Recombinant Protein
Source HEK293 Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Ile 17
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Jul 26 2021