Recombinant Nectin 3 Protein, Human (His Tag) Recombinant Proteins The extracellular domain of human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus The secreted recombinant human PVRL3 comprises 354a.a and has a predicted molecular weight of 39.3 kDa. As a result of glycosylation, rhPVRL3 migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The secreted recombinant human PVRL3 comprises 354a.a and has a predicted molecular weight of 39.3 kDa. As a result of glycosylation, rhPVRL3 migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant Nectin 3 Protein, Human (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-201479

Molecular Weight: The secreted recombinant human PVRL3 comprises 354a.a and has a predicted molecular weight of 39.3 kDa. As a result of glycosylation, rhPVRL3 migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.

Formulation: Recombinant Nectin 3 Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant Nectin 3 Protein, Human (His Tag)
Molecular Weight The secreted recombinant human PVRL3 comprises 354a.a and has a predicted molecular weight of 39.3 kDa. As a result of glycosylation, rhPVRL3 migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
AA Sequence Met 1-Asp 400
Bioactivity Measured by its binding ability in a functional ELISA.1. Immobilized recombinant human PVRL3 at 1 ug/ml (100 ul/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml.2. Immobilized recombinant human PVRL3 at 1 ug/ml (100 ul/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant Nectin 3 Protein was lyophilized from sterile PBS, pH 7.41
Host Species Homo sapiens (Human)
Description The extracellular domain of human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus
Product Background Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
Product Memo CD113 Protein, Human;CDw113 Protein, Human;DKFZp566B0846 Protein, Human;FLJ90624 Protein, Human;nectin-3 Protein, Human;PPR3 Protein, Human;PRR3 Protein, Human;PVRL3 Protein, Human;PVRR3 Protein, Human
Product Type Recombinant Protein
Purity Greater than 98% as determined by SDS-PAGE
Source HEK293 Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Gly 58
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Aug 20 2021