Recombinant MEK1 Protein, Mouse (His & GST Tag) Recombinant Proteins The mouse MAP2K1 (P31938) (Met 1-Ile 393) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus The recombinant mouse MAP2K1/GST chimera consists of 630a.a and has a calculated molecular weight of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant mouse MAP2K1/GST chimera consists of 630a.a and has a calculated molecular weight of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant MEK1 Protein, Mouse (His & GST Tag)

Formulation: Recombinant MEK1 Protein was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.01

Source: Baculovirus-Insect Cells

Product Name Recombinant MEK1 Protein, Mouse (His & GST Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant MEK1 Protein was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.01
Molecular Weight The recombinant mouse MAP2K1/GST chimera consists of 630a.a and has a calculated molecular weight of 71.3 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 95% as determined by SDS-PAGE
Description The mouse MAP2K1 (P31938) (Met 1-Ile 393) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus
Host Species Mouse
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Ile 393
Product Background MEK1, also known as MAP2K1 and MKK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Binding extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause cardiofaciocutaneous syndrome.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Memo MAPKK1 Protein, Mouse;Mek1 Protein, Mouse;MEKK1 Protein, Mouse;Prkmk1 Protein, Mouse
Uniprot Number P31938
Terminal Met
Bioactivity No Kinase Activity