Recombinant Meprin alpha Protein, Mouse (His Tag) Recombinant Proteins The mouse MEP1A (NP_032611.2) (Met 1-Arg 615) was expressed, fused with a polyhistidine tag at the C-terminus The recombinant mouse MEP1A consists of 606a.a after removal of the signal peptide and has a predicted molecular weight of 69 kDa. In SDS-PAGE under reducing conditions, the apparent molecular weight of rm MEP1A is approximately 80-90 kDa due to glycosylation.
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Formulation: The recombinant mouse MEP1A consists of 606a.a after removal of the signal peptide and has a predicted molecular weight of 69 kDa. In SDS-PAGE under reducing conditions, the apparent molecular weight of rm MEP1A is approximately 80-90 kDa due to glycosylation.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant Meprin alpha Protein, Mouse (His Tag)

Formulation: Recombinant Meprin alpha Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant Meprin alpha Protein, Mouse (His Tag)
Product Type Recombinant Protein
Source HEK293 Cells
Formulation Recombinant Meprin alpha Protein was lyophilized from sterile PBS, pH 7.41
Molecular Weight The recombinant mouse MEP1A consists of 606a.a after removal of the signal peptide and has a predicted molecular weight of 69 kDa. In SDS-PAGE under reducing conditions, the apparent molecular weight of rm MEP1A is approximately 80-90 kDa due to glycosylation.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 95% as determined by SDS-PAGE
Description The mouse MEP1A (NP_032611.2) (Met 1-Arg 615) was expressed, fused with a polyhistidine tag at the C-terminus
Host Species Mouse
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Arg 615
Product Background Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein which belongs to thepeptidase M12A family. MEP1A contains oneEGF-like domain, oneMAM domain, and oneMATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).
Memo AI098089 Protein, Mouse;AW107200 Protein, Mouse;Mep-1 Protein, Mouse;Mep-1a Protein, Mouse;Mep1 Protein, Mouse
Terminal Cys 21
Bioactivity Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH, R&D Systems, Catalog # ES007.The specific activity is >400 pmoles/min/ug.(Activation description The proenzyme needs to be activated by Trypsin for an activated form)