Recombinant MBL1 Protein, Mouse (His Tag) Recombinant Proteins The mature form of mouse MBL (NP_034905.1) (Ser 18-Ala 239) was expressed with a N-terminal polyhistidine tag The secreted recombinant mouse MBL consists of 238a.a and has a calculated molecular weight of 25.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 32 kDa protein in SDS-PAGE under reducing conditions.
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Formulation: The secreted recombinant mouse MBL consists of 238a.a and has a calculated molecular weight of 25.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 32 kDa protein in SDS-PAGE under reducing conditions.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant MBL1 Protein, Mouse (His Tag)

Formulation: Recombinant MBL1 Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant MBL1 Protein, Mouse (His Tag)
Product Type Recombinant Protein
Source HEK293 Cells
Formulation Recombinant MBL1 Protein was lyophilized from sterile PBS, pH 7.41
Molecular Weight The secreted recombinant mouse MBL consists of 238a.a and has a calculated molecular weight of 25.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 32 kDa protein in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 92% as determined by SDS-PAGE
Description The mature form of mouse MBL (NP_034905.1) (Ser 18-Ala 239) was expressed with a N-terminal polyhistidine tag
Host Species Mouse
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Ser 18-Ala 239
Product Background Mannose-binding lectin (MBL), also named mannose or mannan-binding protein (MBP), is a C-type lectin which participates in the innate immune system as an activator of the complement system and as opsonin after binding to certain carbohydrate structures on microorganisms and pathogens. Its function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL recognizes carbohydrate patterns found on the surface of a large number of pathogenic micro-organisms including bacteria, viruses, protozoa and fungi. Binding of MBL to a micro-organism results in activation of the lectin pathway of the complement system. Two forms of MBL, MBL-A and MBL-C, were characterized in rodents, rabbits, bovine and rhesus monkeys, whereas only one form was identified in humans, chimpanzees and chickens. The two forms are encoded by two distinct genes named MBL1 and MBL2, which have been identified in many species including the pig. The MBL1 and MBL2 genes encode mannan-binding lectins (MBL) A and C, respectively, that are collagenous lectins (collectins) produced mainly by the liver. The MBL1 gene encodes MBL-A, which has bacteria-binding properties in pigs and rodents but is mutated to a pseudogene in humans and chimpanzees. Deficiency of MBL is probably the most common human immunodeficiency and is associated with an increased risk of mucosally acquired infections including meningococcal disease. MBL could modify disease susceptibility by modulating macrophage interactions with mucosal organisms at the site of initial acquisition.
Memo MBL-A Protein, Mouse;MBP-A Protein, Mouse;S-MBP Protein, Mouse
Terminal His
Bioactivity Using the Octet RED System, the affinity constant (Kd) of mouse MBL bound to biotinylated mannan was 72nM.