Recombinant KEAP1 Protein, Human (His & GST & AVI Tag) Recombinant Proteins The human KEAP1 (Q14145) (Gln 2-Cys 624) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus linked by the AVI tag The recombinant human KEAP1/GST chimera consists of 875a.a and has a calculated molecular weight of 99.2 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
$ $99 In stock
Formulation: The recombinant human KEAP1/GST chimera consists of 875a.a and has a calculated molecular weight of 99.2 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant KEAP1 Protein, Human (His & GST & AVI Tag)

Formulation: Recombinant KEAP1 Protein was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.41

Source: Baculovirus-Insect Cells

Product Name Recombinant KEAP1 Protein, Human (His & GST & AVI Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant KEAP1 Protein was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.41
Molecular Weight The recombinant human KEAP1/GST chimera consists of 875a.a and has a calculated molecular weight of 99.2 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 90% as determined by SDS-PAGE
Description The human KEAP1 (Q14145) (Gln 2-Cys 624) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus linked by the AVI tag
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Gln 2-Cys 624
Product Background Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Memo INRF2 Protein, Human;KEAP-1 Protein, Human;KLHL19 Protein, Human
Uniprot Number Q14145
Terminal Met
Bioactivity Please contact us for detailed information