Recombinant IL-35 (IL12A & EBI3 Heterodimer) Protein, Human Recombinant Proteins The p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1, The human IL27B, (NP_005746.2) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified The recombinant human IL12A&IL27B comprises 905 (447+458)a.a and has a calculated molecular weight of 102 (50.7+ 51.3) KDa. The apparent molecular weight of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
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Formulation: The recombinant human IL12A&IL27B comprises 905 (447+458)a.a and has a calculated molecular weight of 102 (50.7+ 51.3) KDa. The apparent molecular weight of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant IL-35 (IL12A & EBI3 Heterodimer) Protein, Human

Formulation: Recombinant IL-35 (IL12A & EBI3 Heterodimer) Protein was lyophilized from sterile PBS, pH 7.4.1

Source: HEK293 Cells

Product Name Recombinant IL-35 (IL12A & EBI3 Heterodimer) Protein, Human
Product Type Recombinant Protein
Source HEK293 Cells
Formulation Recombinant IL-35 (IL12A & EBI3 Heterodimer) Protein was lyophilized from sterile PBS, pH 7.4.1
Molecular Weight The recombinant human IL12A&IL27B comprises 905 (447+458)a.a and has a calculated molecular weight of 102 (50.7+ 51.3) KDa. The apparent molecular weight of the recombinant human IL12A&IL27B is approximately 60 & 63 KDa respectively in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity (59.8+33.3)% as determined by SDS-PAGE
Description The p35 subunit of human IL12, termed as IL12A (NP_000873.2) (Met1-Ser219) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1, The human IL27B, (NP_005746.2) (Met1-Lys229) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met1-Ser219
Product Background IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.
Memo CLMF Protein, Human;IL-12A Protein, Human;NFSK Protein, Human;NKSF1 Protein, Human;P35 Protein, Human
Terminal Arg 23 & Arg 21
Bioactivity Please contact us for detailed information