Recombinant IL-23 P19 Protein, Rat (His Tag) Recombinant Proteins The rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1, The rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified The recombinant rat IL23A & the rat IL12B comprises 510 (186+324)a.a and has a calculated molecular weight of 58.2 (21+37.2) KDa. The apparent molecular weight of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
$ $99 In stock
Formulation: The recombinant rat IL23A & the rat IL12B comprises 510 (186+324)a.a and has a calculated molecular weight of 58.2 (21+37.2) KDa. The apparent molecular weight of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant IL-23 P19 Protein, Rat (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-202075

Molecular Weight: The recombinant rat IL23A & the rat IL12B comprises 510 (186+324)a.a and has a calculated molecular weight of 58.2 (21+37.2) KDa. The apparent molecular weight of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.

Formulation: Recombinant IL-23 P19 Protein was lyophilized from sterile PBS, pH 7.4.1

Source: HEK293 Cells

Product Name Recombinant IL-23 P19 Protein, Rat (His Tag)
Molecular Weight The recombinant rat IL23A & the rat IL12B comprises 510 (186+324)a.a and has a calculated molecular weight of 58.2 (21+37.2) KDa. The apparent molecular weight of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
AA Sequence Met1-Ala196
Bioactivity Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 2-15 ng/mL.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant IL-23 P19 Protein was lyophilized from sterile PBS, pH 7.4.1
Host Species Rat
Description The rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1, The rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified
Product Background IL-23, which is mainly secreted by antigen-presenting cells, is a member of the IL-12 family, which includes IL-12, IL-27, and IL-35[1]. IL-23 is a heterodimeric cytokine, comprised a unique p19 subunit and p4 subunit, the latter of which is shared with IL-12. The receptor for IL-23 consists of IL-23R and IL-12Rβ1, the latter of which is also characteristic of IL-12. IL-23 is essential for Th17 differentiation, expansion, and survival by binding to its receptor, thereby activating the signaling pathway [11,12]. Many studies revealed that the IL-23/Th17 pathway is implicated in the pathophysiology of various autoimmune diseases, such as autoimmune arthritis[13], primary biliary cirrhosis[14], and inflammatory bowel disease[15].Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Product Type Recombinant Protein
Purity (17.9+53.5+2.9+23.8)% as determined by SDS-PAGE
Source HEK293 Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Val 22 & Met 23
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Aug 20 2021