Recombinant Iduronate 2 sulfatase Protein, Human (His Tag) Recombinant Proteins A DNA sequence encoding human IDS precursor (NP_000193.1) (Met 1-Pro 550) was expressed with a C-terminal polyhistidine tag The secreted recombinant human IDS with the propeptide consists of 536a.a after removal of the signal peptide and has a predicted molecular weight of 61 kDa. In SDS-PAGE under reducing conditions, it migrates as several bands with apparent molecular weight between 85-95 KDa due to glycosylation.
$ $99 In stock
Formulation: The secreted recombinant human IDS with the propeptide consists of 536a.a after removal of the signal peptide and has a predicted molecular weight of 61 kDa. In SDS-PAGE under reducing conditions, it migrates as several bands with apparent molecular weight between 85-95 KDa due to glycosylation.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant Iduronate 2 sulfatase Protein, Human (His Tag)

Formulation: Recombinant Iduronate 2 sulfatase Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant Iduronate 2 sulfatase Protein, Human (His Tag)
Product Type Recombinant Protein
Source HEK293 Cells
Formulation Recombinant Iduronate 2 sulfatase Protein was lyophilized from sterile PBS, pH 7.41
Molecular Weight The secreted recombinant human IDS with the propeptide consists of 536a.a after removal of the signal peptide and has a predicted molecular weight of 61 kDa. In SDS-PAGE under reducing conditions, it migrates as several bands with apparent molecular weight between 85-95 KDa due to glycosylation.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 87% as determined by SDS-PAGE
Description A DNA sequence encoding human IDS precursor (NP_000193.1) (Met 1-Pro 550) was expressed with a C-terminal polyhistidine tag
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Pro 550
Product Background Iduronate 2-Sulfatase, also known as IDS, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. The human Iduronate 2-Sulfatase/IDS consists of a signal peptide, a pro peptide and a mature chain that may be further processed into two chains. Among the identified 18 human sulfatases, Iduronate 2-Sulfatase/IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG), heparan sulfate and dermatan sulfate. Multiple mutations in this X-chromosome localized gene result in Iduronate 2-Sulfatase/IDS enzymatic deficiency, and lead to the sex-linked Mucopolysaccharidosis Type II (MPS II ), also known as Hunter Syndrome characterized by the lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe due to the level of Iduronate 2-Sulfatase/IDS enzyme. Retroviral-mediated Iduronate 2-Sulfatase/IDS gene transfer into lymphoid cells would be a promising gene therapeutic strategy.
Memo IDS Protein, Human;MPS2 Protein, Human;SIDS Protein, Human
Terminal Ser 26
Bioactivity Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).The specific activity is > 1.0 pmoles/min/ug.