Recombinant HtrA2 / Omi Protein, Human (His Tag) Recombinant Proteins The mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) was expressed, with a polyhistide tag at the C-terminus The recombinant human HTRA2 consisting of 336a.a and has a calculated molecular weight of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.
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Formulation: The recombinant human HTRA2 consisting of 336a.a and has a calculated molecular weight of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.
Source: E. coli
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant HtrA2 / Omi Protein, Human (His Tag)

Formulation: Recombinant HtrA2 / Omi Protein was lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.81

Source: E. coli

Product Name Recombinant HtrA2 / Omi Protein, Human (His Tag)
Product Type Recombinant Protein
Source E. coli
Formulation Recombinant HtrA2 / Omi Protein was lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.81
Molecular Weight The recombinant human HTRA2 consisting of 336a.a and has a calculated molecular weight of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.
Purity Greater than 87% as determined by SDS-PAGE
Description The mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) was expressed, with a polyhistide tag at the C-terminus
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Ala 134-Glu 458
Product Background Serine protease HTRA2, also known as high temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI and HTRA2, is a single-pass membrane protein which belongs to thepeptidase S1B family. HTRA2 contains onePDZ (DHR) domain. HTRA2 is a serine protease that shows proteolytic activity against a non-specific substrate beta-casein. It promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. HTRA2 cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2of HTRA2 seems to be proteolytically inactive. Defects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) which is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa.
Memo OMI Protein, Human;PARK13 Protein, Human;PRSS25 Protein, Human
Uniprot Number O43464
Terminal Met
Bioactivity Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10 1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.