Recombinant DR5 Protein, Human (His Tag) Recombinant Proteins The human TNFRSF10B (NP_003833.3) extracellular domain (Met 1-Glu 182) was expressed, fused with a polyhistidine tag at the C-terminus The recombinant human TNFRSF10B consists of 138a.a and has a predicted molecular weight of 15.8 kDa. As a result of glycosylation, the apparent molecular weight of rhTNFRSF10B is approxiamtely 20-22 kDa in SDS-PAGE under reducing conditions.
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Formulation: The recombinant human TNFRSF10B consists of 138a.a and has a predicted molecular weight of 15.8 kDa. As a result of glycosylation, the apparent molecular weight of rhTNFRSF10B is approxiamtely 20-22 kDa in SDS-PAGE under reducing conditions.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant DR5 Protein, Human (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-203259

Molecular Weight: The recombinant human TNFRSF10B consists of 138a.a and has a predicted molecular weight of 15.8 kDa. As a result of glycosylation, the apparent molecular weight of rhTNFRSF10B is approxiamtely 20-22 kDa in SDS-PAGE under reducing conditions.

Formulation: Recombinant DR5 Protein was lyophilized from sterile PBS, pH 7.41

Source: HEK293 Cells

Product Name Recombinant DR5 Protein, Human (His Tag)
Molecular Weight The recombinant human TNFRSF10B consists of 138a.a and has a predicted molecular weight of 15.8 kDa. As a result of glycosylation, the apparent molecular weight of rhTNFRSF10B is approxiamtely 20-22 kDa in SDS-PAGE under reducing conditions.
AA Sequence Met 1-Glu 182
Bioactivity 1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 ug/ml (100 ul/well) can bind biotinylated TNFSF10 with a linear range of 0.625-20 ng/ml.2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 5-25 ng/mL in the presence of 10 ng/mL Recombinant Human TRAIL/TNFSF10.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant DR5 Protein was lyophilized from sterile PBS, pH 7.41
Host Species Homo sapiens (Human)
Description The human TNFRSF10B (NP_003833.3) extracellular domain (Met 1-Glu 182) was expressed, fused with a polyhistidine tag at the C-terminus
Product Background Tumor necrosis factor receptor superfamily, member 1b, official symbol TNFRSF1B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF1/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF1B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF1B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF1B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF1B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Product Memo CD262 Protein, Human;DR5 Protein, Human;KILLER Protein, Human;KILLER/DR5 Protein, Human;TRAIL-R2 Protein, Human;TRAILR2 Protein, Human;TRICK2 Protein, Human;TRICK2A Protein, Human;TRICK2B Protein, Human;TRICKB Protein, Human;ZTNFR9 Protein, Human
Product Type Recombinant Protein
Purity Greater than 95% as determined by SDS-PAGE
Source HEK293 Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Ile 56
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Aug 20 2021