Recombinant DLL1 Protein, Rat (His Tag) Recombinant Proteins The rat DLL1 (EDL99825.1) extracellular domain (Met 1-Ser 534) was expressed, fused with a polyhistidine tag at the C-terminus The recombinant rat DLL1 comprises 746a.a and has a predicted molecular weight of 81.7 kDa.
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Formulation: The recombinant rat DLL1 comprises 746a.a and has a predicted molecular weight of 81.7 kDa.
Source: HEK293 Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant DLL1 Protein, Rat (His Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog Number: BT-203268

Molecular Weight: The recombinant rat DLL1 comprises 746a.a and has a predicted molecular weight of 81.7 kDa.

Formulation: Recombinant DLL1 Protein was lyophilized from sterile PBS, 20% glycerol, pH 7.41

Source: HEK293 Cells

Product Name Recombinant DLL1 Protein, Rat (His Tag)
Molecular Weight The recombinant rat DLL1 comprises 746a.a and has a predicted molecular weight of 81.7 kDa.
AA Sequence Met 1-Ser 534
Bioactivity 1. Measured by its ability to bind human NOTCH1 in a functional ELISA.2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.The ED50 for this effect is typically 2-10 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant DLL1 Protein was lyophilized from sterile PBS, 20% glycerol, pH 7.41
Host Species Rat
Description The rat DLL1 (EDL99825.1) extracellular domain (Met 1-Ser 534) was expressed, fused with a polyhistidine tag at the C-terminus
Product Background Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains, acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM1. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N- or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.
Product Type Recombinant Protein
Purity Greater than 65% as determined by SDS-PAGE
Source HEK293 Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Gln 18
Uniprot No G3V7W7
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Aug 20 2021