Recombinant DNMT2 Protein, Human (GST Tag) Recombinant Proteins The human TRDMT1 isoform a (NP_004403.1) (Met 1-Glu 391) was fused with the GST tag at the N-terminus The recombinant human TRDMT1/GST chimera consists of 616a.a and predicts a molecular weight of 71 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant human TRDMT1/GST chimera consists of 616a.a and predicts a molecular weight of 71 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant DNMT2 Protein, Human (GST Tag)

Formulation: Recombinant DNMT2 Protein was lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, pH 8.01

Source: Baculovirus-Insect Cells

Product Name Recombinant DNMT2 Protein, Human (GST Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant DNMT2 Protein was lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, pH 8.01
Molecular Weight The recombinant human TRDMT1/GST chimera consists of 616a.a and predicts a molecular weight of 71 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 94% as determined by SDS-PAGE
Description The human TRDMT1 isoform a (NP_004403.1) (Met 1-Glu 391) was fused with the GST tag at the N-terminus
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Glu 391
Product Background DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 1 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.
Memo DMNT2 Protein, Human;DNMT2 Protein, Human;MHSAIIP Protein, Human;PUMET Protein, Human;RNMT1 Protein, Human
Terminal Met
Bioactivity Please contact us for detailed information