Recombinant CDK1 Protein, Mouse (His & GST Tag) Recombinant Proteins The mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus The recombinant mouse CDK1/GST chimera consists of 534a.a and has a calculated molecular weight of 61.9 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant mouse CDK1/GST chimera consists of 534a.a and has a calculated molecular weight of 61.9 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant CDK1 Protein, Mouse (His & GST Tag)

Formulation: Recombinant CDK1 ProteinSupplied as sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0.1

Source: Baculovirus-Insect Cells

Product Name Recombinant CDK1 Protein, Mouse (His & GST Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant CDK1 ProteinSupplied as sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0.1
Molecular Weight The recombinant mouse CDK1/GST chimera consists of 534a.a and has a calculated molecular weight of 61.9 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 85% as determined by SDS-PAGE
Description The mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus
Host Species Mouse
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met1-Met297
Product Background CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
Memo Cdc2 Protein, Mouse;CDC2&gt Protein, Mouse;Cdc2a Protein, Mouse;p34&lt Protein, Mouse
Uniprot Number P11440
Terminal Met
Bioactivity Kinase activity untested