Recombinant CDK2 Protein, Human (His Tag) Recombinant Proteins The human cyclin-dependent kinase 2 isoform 1 (NP_001789.2) (Met 1-Leu 298) was expressed, fused with a polyhistidine tag at the C-terminus The recombinant human CDK2 consists of 308a.a and predicts a molecular weight of 35 kDa. The apparent molecular weight of rhCDK2 is approximately 33 kDa in SDS-PAGE under reducing conditions.
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Formulation: The recombinant human CDK2 consists of 308a.a and predicts a molecular weight of 35 kDa. The apparent molecular weight of rhCDK2 is approximately 33 kDa in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant CDK2 Protein, Human (His Tag)

Formulation: Recombinant CDK2 Protein was lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol1

Source: Baculovirus-Insect Cells

Product Name Recombinant CDK2 Protein, Human (His Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant CDK2 Protein was lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol1
Molecular Weight The recombinant human CDK2 consists of 308a.a and predicts a molecular weight of 35 kDa. The apparent molecular weight of rhCDK2 is approximately 33 kDa in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 85% as determined by SDS-PAGE
Description The human cyclin-dependent kinase 2 isoform 1 (NP_001789.2) (Met 1-Leu 298) was expressed, fused with a polyhistidine tag at the C-terminus
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Leu 298
Product Background CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. CDK2 associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. CDK2 is involved in the control of the cell cycle. It also interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Memo CDKN2 Protein, Human;p33(CDK2) Protein, Human
Terminal Met
Bioactivity No Kinase Activity