Recombinant Brk Protein, Mouse (His & GST Tag) Recombinant Proteins The mouse PTK6 (Q64434) (Met1-Val451) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus The recombinant mouse PTK6/GST chimera consists of 688a.a and has a calculated molecular weight of 79.8 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant mouse PTK6/GST chimera consists of 688a.a and has a calculated molecular weight of 79.8 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant Brk Protein, Mouse (His & GST Tag)

Formulation: Recombinant Brk ProteinSupplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol1

Source: Baculovirus-Insect Cells

Product Name Recombinant Brk Protein, Mouse (His & GST Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant Brk ProteinSupplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol1
Molecular Weight The recombinant mouse PTK6/GST chimera consists of 688a.a and has a calculated molecular weight of 79.8 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 90% as determined by SDS-PAGE
Description The mouse PTK6 (Q64434) (Met1-Val451) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus
Host Species Mouse
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met1-Val451
Product Background Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.
Memo BRK Protein, Mouse;Sik Protein, Mouse;tks Protein, Mouse;Tksk Protein, Mouse
Uniprot Number Q64434
Terminal Met
Bioactivity The specific activity was determined to be 5 nmol/min/mg using poly [Glu, Tyr] 4 1 as substrate.