Recombinant BTK Protein, Human (His Tag) Recombinant Proteins The human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag The secreted recombinant human BTK consists of 670a.a and predicts a molecular weight of 77.8 KDa.
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Formulation: The secreted recombinant human BTK consists of 670a.a and predicts a molecular weight of 77.8 KDa.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant BTK Protein, Human (His Tag)

Formulation: Recombinant BTK ProteinSupplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0.1

Source: Baculovirus-Insect Cells

Product Name Recombinant BTK Protein, Human (His Tag)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant BTK ProteinSupplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0.1
Molecular Weight The secreted recombinant human BTK consists of 670a.a and predicts a molecular weight of 77.8 KDa.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 85% as determined by SDS-PAGE
Description The human BTK (NP_000052.1) (Met 1-Ser 659) was expressed with a C-terminal polyhistidine tag
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Ser 659
Product Background Bruton's tyrosine kinase (or BTK) is a type of kinase protein expressed in B lymphocytes and T cells. BTK contains a PH domain which binds phosphatidylinositol(3,4,5)-trisphosphate (PIP3). After binding to PIP3, BTK is induced to phosphorylate phospholipase C, which in turn hydrolyzes PIP2 into two second messagers, IP3 and DAG, which then modulate the activity of downstream proteins during B-cell signaling. Btk is also found implicated in the primary immunodeficiency disease X-linked agammaglobulinemia(Bruton's agammaglobulinemia). BTK played a key role in B-cell maturation as well as mast cell activation through the high-affinity IgE receptor. Patients with X-linked agammaglobulinemia have normal pre-B cell populations in their bone marrow but these B-cells can not mature and enter the circulation.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Memo AGMX1 Protein, Human;AT Protein, Human;ATK Protein, Human;BPK Protein, Human;IMD1 Protein, Human;PSCTK1 Protein, Human;XLA Protein, Human
Terminal Met
Bioactivity 1. The specific activity was determined to be >50 nmol/min/mg using Poly(Glu,Tyr)4 1 peptide as substrate.2. Measured by its ability to bind human BLNK in a functional ELISA.