Recombinant AMPK alpha 1 Protein, Human Recombinant Proteins The human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, The human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2, The human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular weight of 160 (38+30+92) KDa. The apparent molecular weight is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
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Formulation: The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular weight of 160 (38+30+92) KDa. The apparent molecular weight is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant AMPK alpha 1 Protein, Human

Formulation: Recombinant AMPK alpha 1 ProteinSupplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.41

Source: Baculovirus-Insect Cells

Product Name Recombinant AMPK alpha 1 Protein, Human
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant AMPK alpha 1 ProteinSupplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.41
Molecular Weight The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB1 / PRKAA1) has a calculated molecular weight of 160 (38+30+92) KDa. The apparent molecular weight is approximately 38, 40 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 94% as determined by SDS-PAGE
Description The human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1, The human PRKAB1 (Q9Y478) (Met 1-Ile 270), constructed the plasmid 2, The human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie
Host Species Homo sapiens (Human)
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Met 1-Pro 331
Product Background The PRKAA1 gene encodes the catalytic alpha-subunit of 5' AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor that maintains energy homeostasis within the cell and is activated when the AMP/ATP ratio increases. Moreover, AMPK has been shown to be involved in inhibiting tumour growth and metastasis, and has also been implicated in the pathology of neurodegenerative and cardiac disorders. All 5 tested tag SNPs of the PRKAA1 gene (rs1336177, rs154268, rs385486, rs688293, and rs174991) were significantly associated with gastric cancer.An inhibitor of PRKAA1 promoted the secretion of IL6, whereas an agonist of PRKAA1 suppressed the production of IL6.
Memo AMPK Protein, Human;AMPKa1 Protein, Human
Uniprot Number P54619
Terminal Met & Met & His
Bioactivity The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.