Recombinant AKT3 Protein, Mouse (aa 106-479) Recombinant Proteins The AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed and purified The recombinant mouse AKT3 consists of 376a.a and predicts a molecular weight of 43.4 KDa. It migrates as an approximately 46 KDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant mouse AKT3 consists of 376a.a and predicts a molecular weight of 43.4 KDa. It migrates as an approximately 46 KDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant AKT3 Protein, Mouse (aa 106-479)

Formulation: Recombinant AKT3 ProteinSupplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.41

Source: Baculovirus-Insect Cells

Product Name Recombinant AKT3 Protein, Mouse (aa 106-479)
Product Type Recombinant Protein
Source Baculovirus-Insect Cells
Formulation Recombinant AKT3 ProteinSupplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.41
Molecular Weight The recombinant mouse AKT3 consists of 376a.a and predicts a molecular weight of 43.4 KDa. It migrates as an approximately 46 KDa band in SDS-PAGE under reducing conditions.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Purity Greater than 95% as determined by SDS-PAGE
Description The AKT3 (Q9WUA6-1) (Ala106-Glu479) was expressed and purified
Host Species Mouse
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
AA Sequence Ala106-Glu479
Product Background v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt kinase family, share extensive structural similarity and perform common as well as unique functions within cells. The Akt signaling cascade initiates at the cell surface when growth factors or other extracellular stimuli activate phosphoinositide 3-kinase (PI3K). AKT3 was discovered to be the predominant isoform activated in sporadic melanomas. Levels of activity increased during melanoma progression with metastatic melanomas having the highest activity. Although mechanisms of AKT3 activation remain to be fully characterized, overexpression of AKT3 and decreased PTEN activity play important roles in this process. Targeted reduction of AKT3 activity decreased survival of melanoma tumor cells leading to inhibition of tumor development, which may be therapeutically effective for shrinking tumors in melanoma patients. AKT2 and AKT3 play an important role in the viability of human malignant glioma cells. Targeting AKT2 and AKT3 may hold promise for the treatment of patients with gliomas.
Memo AI851531 Protein, Mouse;D930002M15Rik Protein, Mouse;Nmf350 Protein, Mouse
Uniprot Number Q9WUA6
Terminal Gly
Bioactivity The specific activity was determined to be 20 nmol/min/mg using synthetic GSK3-derived peptide (R11-SGRARTSSFAEPGGK) as substrate.