Recombinant ALK4/ACVR1B Protein, Human (His & Fc Tag)
Recombinant Proteins
The human ACVR1B (NP_004293.1) extracellular domain (Met 1-Glu 126) was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus
The recombinant human ALK4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 351a.a and has a predicted molecular weight of 39.6 kDa. In SDS-PAGE under reducing conditions, rhALK4/Fc monomer migrates as an approximately 46 kDa band due to glycosylation, with ~15% free Fc fragment.
Formulation:
The recombinant human ALK4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 351a.a and has a predicted molecular weight of 39.6 kDa. In SDS-PAGE under reducing conditions, rhALK4/Fc monomer migrates as an approximately 46 kDa band due to glycosylation, with ~15% free Fc fragment.
Source:
HEK293 Cells
Usage:
Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use
Recombinant ALK4/ACVR1B Protein, Human (His & Fc Tag)
Formulation: Recombinant ALK4/ACVR1B Protein was lyophilized from sterile PBS, pH 7.41
Source: HEK293 Cells
| Product Name | Recombinant ALK4/ACVR1B Protein, Human (His & Fc Tag) |
|---|---|
| Product Type | Recombinant Protein |
| Source | HEK293 Cells |
| Formulation | Recombinant ALK4/ACVR1B Protein was lyophilized from sterile PBS, pH 7.41 |
| Molecular Weight | The recombinant human ALK4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 351a.a and has a predicted molecular weight of 39.6 kDa. In SDS-PAGE under reducing conditions, rhALK4/Fc monomer migrates as an approximately 46 kDa band due to glycosylation, with ~15% free Fc fragment. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Purity | Greater than 80% as determined by SDS-PAGE |
| Description | The human ACVR1B (NP_004293.1) extracellular domain (Met 1-Glu 126) was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus |
| Host Species | Homo sapiens (Human) |
| Usage | Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use |
| Storage | Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| AA Sequence | Met 1-Glu 126 |
| Product Background | ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination. |
| Memo | ACTRIB Protein, Human;ACVRLK4 Protein, Human;ALK4 Protein, Human;SKR2 Protein, Human |
| Terminal | Ser 24 |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human TDGF1 at 2 ug/ml (100 ul/well) can bind human ALK-4 with a linear range of 0.0068-0.16 ug/ml. |