Recombinant KEAP1 Protein, Human (His & GST Tag) Recombinant Proteins The human KEAP1 (NP_036421.2) (Gln2-Cys624) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus The recombinant human KEAP1/GST chimera consists of 861a.a and has a calculated molecular weight of 97.5 kDa. The recombinant protein migrates as an approximately 109 kDa band in SDS-PAGE under reducing conditions.
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Formulation: The recombinant human KEAP1/GST chimera consists of 861a.a and has a calculated molecular weight of 97.5 kDa. The recombinant protein migrates as an approximately 109 kDa band in SDS-PAGE under reducing conditions.
Source: Baculovirus-Insect Cells
Usage: Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant KEAP1 Protein, Human (His & GST Tag)

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog No: bt-213015

Molecular Weight: The recombinant human KEAP1/GST chimera consists of 861a.a and has a calculated molecular weight of 97.5 kDa. The recombinant protein migrates as an approximately 109 kDa band in SDS-PAGE under reducing conditions.

Formulation: Recombinant KEAP1 Protein was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.1

Source: Baculovirus-Insect Cells

Product Name Recombinant KEAP1 Protein, Human (His & GST Tag)
Molecular Weight The recombinant human KEAP1/GST chimera consists of 861a.a and has a calculated molecular weight of 97.5 kDa. The recombinant protein migrates as an approximately 109 kDa band in SDS-PAGE under reducing conditions.
AA Sequence Gln2-Cys624
Bioactivity Please contact us for detailed information
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Recombinant KEAP1 Protein was lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.1
Host Species Homo sapiens (Human)
Description The human KEAP1 (NP_036421.2) (Gln2-Cys624) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus
Product Background Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Product Memo INRF2 Protein, Human;KEAP-1 Protein, Human;KLHL19 Protein, Human
Product Type Recombinant Protein
Purity Greater than 85% as determined by SDS-PAGE
Source Baculovirus-Insect Cells
Storage Store recombinant protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Terminal Met
Usage Our products are for research use only. This product is not intended or approved for human, diagnostics or veterinary use