Recombinant Human EGF Protein, His Tag Recombinant Proteins Recombinant Human EGF was purified from the endosperm tissue of barley grain (Hordeum vulgare)
$ $99 In stock
Formulation:
Source: Barley grain (Hordeum vulgare)
Usage: Our recombinant proteins are For Research Use Only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant Human EGF Protein, His Tag

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog No: bt-215770

Formulation: PBS pH 7.2, sterile filtered

Source: Barley grain (Hordeum vulgare)

Product Name Recombinant Human EGF Protein, His Tag
Bioactivity Recombinant human EGF is assayed by measuring its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using EGF is typically< 0.1 ng/ml, corresponding to a specific activity>10 x10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.
Description Recombinant Human EGF was purified from the endosperm tissue of barley grain (Hordeum vulgare)
Endotoxin Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg)
Formulation PBS pH 7.2, sterile filtered
Host Species Homo sapiens (human)
Product Background Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture and to be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide-bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues is conserved.
Product Type Recombinant Protein
Purity Greater than 95% by SDS-PAGE gel analysis.
Reconstitution Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA)
Source Barley grain (Hordeum vulgare)
Storage The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Synonym EGF, epidermal growth factor, epidermal growth factor (beta urogastrone), pro-epidermal growth factor, beta-urogastrone, URG, HOMG4
Uniprot No P01133
Usage Our recombinant proteins are For Research Use Only. This product is not intended or approved for human, diagnostics or veterinary use