Recombinant Human D4GDI Protein Recombinant Proteins
$ $99 In stock
Formulation:
Source: E.coli
Usage: Our recombinant proteins are For Research Use Only. This product is not intended or approved for human, diagnostics or veterinary use

Recombinant Human D4GDI Protein

The product is for non-human research only. Not for therapeutic or veterinary use.

Catalog No: bt-220328

Formulation: D4-GDI protein at 0.1mg/ml in 50mM Tris-HCl, pH7.5 and 10mM L-Glutathione (reduced).

Source: E.coli

Product Name Recombinant Human D4GDI Protein
Formulation D4-GDI protein at 0.1mg/ml in 50mM Tris-HCl, pH7.5 and 10mM L-Glutathione (reduced).
Host Species Homo sapiens (Human)
Product Background D4-GDI belongs to the family of Rho-GDIs or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of RhoGDI-1, D4 GDI/RhoGDI-2 and RhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and is involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas-mediated apoptosis. D4-GDI is expressed only in hematopoietic tissues, mainly in B and T cells, unlike RhoGDI which is expressed ubiquitously. D4-GDI plays a role in the regulation of lymphocyte activation, survival, and responsiveness. Expression of D4-GDI is also observed in cells of nonhematopoietic neoplasms, including ovarian and bladder cancer cells, suggestive of D4-GDI’s role in progression of human tumors. D4-GDI is expressed in human breast cancer cells. In different human breast cancer cell lines that were studied, cell lines with high invasive activities, for example MDA-MB-231, expressed higher D4-GDI than did weakly invasive and noninvasive cell lines. Intentional disruption of D4-GDI by steadily expressing small interfering RNA (siRNA) against D4-GDI transcripts successfully blocks the motility and invasive potential of MDA-MB-231 cells in vitro. Cells deficient of D4-GDI revert to a normal breast epithelial phenotype characterized by the formation of cysts with a central lumen when grown on Matrigel. D4-GDI reduction is linked to a down-regulation of the cell surface protein ?1-integrin and a loss of cell-matrix adhesion, suggestive of D4-GDI induction of cell invasion by controlling ?1-integrin expression. The expression of ?1-integrin and invasive activities are restored once there’s restitution of D4-GDI expression to the levels found in the parental MDA-MB-231 cells. Therefore it is suggested that D4-GDI may play a significant role in the regulation of breast cancer cell invasiveness.
Product Memo D4GDI Human D4-GDI Human Recombinant
Product Type Recombinant Protein
Purity >90% as determined by SDS-PAGE.
Source E.coli
Stability Recombinant protein is stable up to 1 year at -80℃
Storage Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid repeated freeze-thaw cycles.
Synonym D4-GDI, D4GDI
Uniprot No P52566
Usage Our recombinant proteins are For Research Use Only. This product is not intended or approved for human, diagnostics or veterinary use
Last Modified Mar 08 2019