PRSS7 Human

Protease Serine 7 Human Recombinant

Shipped with Ice Packs

In Stock

Cat. No.

BT4611

Source

Escherichia Coli.

Synonyms

PRSS7,ENTK,Protease, Serine, 7 (Enterokinase), Transmembrane Protease, Serine 15, Serine Protease 7, Enteropeptidase, EC 3.4.21.9, Transmembrane Protease Serine 15,Enterokinase Catalytic Subunit, Proenterokinase, Enterokinase, EC 3.4.21.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Quick Inquiry

Description

Biological Function and Mechanism

PRSS7 catalyzes the hydrolysis of trypsinogen to trypsin, initiating pancreatic enzyme activation:

\text{Trypsinogen} \xrightarrow{\text{PRSS7}} \text{Trypsin} + \text{Hexapeptide (Val-Asp-Asp-Asp-Asp-Lys)}

This reaction triggers downstream activation of chymotrypsinogen, procarboxypeptidases, and proelastases .

Key Features:

Substrate Specificity: Requires the sequence Asp-Asp-Asp-Asp-Lys .
Localization: Membrane-bound via heavy chain; resistant to soybean trypsin inhibitor .

Clinical Significance

Enteropeptidase Deficiency:

Symptoms: Severe diarrhea, failure to thrive, hypoproteinemia .
Treatment: Oral pancreatic enzyme supplementation .

Disease Associations:

Acute Pancreatitis: Reflux into pancreatic ducts may prematurely activate zymogens .
Alzheimer’s Disease: Genetic linkage to chromosome 21q21 SNPs .

Research Applications

Proteomics:

LC-MS/MS studies (e.g., PRSS37 knockout mice) revealed perturbed proteomes in male reproductive tracts, highlighting PRSS7’s role in fertility .

Virology:

Enhances influenza A virus infection by activating trypsinogen to process viral hemagglutinin .

Biotechnology:

Used to cleave fusion proteins (e.g., His-tagged proteins) due to high specificity .

Product Specs

Introduction

Protease Serine 7, alternatively referred to as PRSS7, plays a crucial role in initiating the activation of pancreatic proteolytic proenzymes, including trypsinogen, chymotrypsinogen, and procarboxypeptidase A. Specifically, PRSS7 catalyzes the conversion of trypsinogen into its active form, trypsin. Subsequently, trypsin triggers the activation of other proenzymes, such as chymotrypsinogen, procarboxypeptidases, and proelastases.

Description

Recombinant human PRSS7, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 237 amino acids (residues 785-1019). This protein has a molecular weight of 26.4 kDa. The purification of PRSS7 is achieved through proprietary chromatographic techniques.

Physical Appearance

A clear, colorless solution that has been sterilized by filtration.

Formulation

The PRSS7 protein solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0) and 10% glycerol.

Stability

For short-term storage (2-4 weeks), the product should be stored at 4°C.

For extended storage, it is recommended to freeze the product at -20°C.

To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is advised.

Repeated freeze-thaw cycles should be avoided.

Purity

The purity of the protein is determined to be greater than 85% as assessed by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MAIVGGSNAK EGAWPWVVGL YYGGRLLCGA SLVSSDWLVS AAHCVYGRNL EPSKWTAILG LHMKSNLTSP QTVPRLIDEI VINPHYNRRR KDNDIAMMHL EFKVNYTDYI QPICLPEENQ VFPPGRNCSI AGWGTVVYQG TTANILQEAD VPLLSNERCQ QQMPEYNITE NMICAGYEEG GIDSCQGDSG GPLMCQENNR WFLAGVTSFG YKCALPNRPG VYARVSRFTE WIQSFLH

Product Science Overview

Structure and Function

PRSS7 catalyzes the conversion of trypsinogen to trypsin. Trypsin, in turn, activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. This cascade of enzyme activation is essential for the digestion of proteins in the small intestine. The precise regulation of these enzymes is critical for maintaining digestive health and preventing conditions such as pancreatitis.

Recombinant Production

The recombinant production of proteases, including PRSS7, has become a significant focus due to the increasing demand for these enzymes in various industries. Recombinant proteases are produced using microbial systems, which offer advantages such as high-level expression and compatibility with downstream applications. The production process involves the insertion of the gene encoding PRSS7 into a suitable host organism, such as Escherichia coli or Bacillus species, which then expresses the enzyme.

Applications

Recombinant PRSS7 has several applications in biotechnology and medicine. In the medical field, it is used in research related to digestive enzymes and their role in various diseases. In biotechnology, PRSS7 is utilized in the production of other enzymes and in processes that require precise proteolytic activity.

Advances in Research

Recent advancements in protease engineering, including rational design and directed evolution, have improved the efficiency and stability of recombinant proteases. These techniques allow for the modification of PRSS7 to enhance its properties, making it more suitable for industrial and medical applications.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

PRSS7 Human

Description

Biological Function and Mechanism

Key Features:

Clinical Significance

Enteropeptidase Deficiency:

Disease Associations:

Research Applications

Proteomics:

Virology:

Biotechnology:

Product Specs

Product Science Overview

Quick Inquiry

Category

Service