Os07g0544800 Antibody

Target Protein Overview

Oxygen-evolving enhancer protein 3 (OEE3) is a subunit of the Photosystem II (PSII) oxygen-evolving complex (OEC), which stabilizes manganese clusters essential for water splitting during photosynthesis. In rice, this protein is encoded by the Os07g0544800 gene. Dysregulation of OEE3 impacts photosynthetic efficiency and stress responses in plants .

Validation and Cross-Reactivity

The antibody’s specificity was confirmed via immunoblot assays, showing reactivity with the ~23 kDa OEE3 protein in rice lysates. Cross-reactivity studies indicate recognition of homologous OEE3 proteins in maize (Zea mays), grapevine (Vitis vinifera), and soybean (Glycine max), suggesting conserved epitopes across plant species .

Key Validation Data:

• Western Blot: Clear band at expected molecular weight in rice chloroplast extracts .

• Immunohistochemistry: Localizes to chloroplasts in rice leaf tissue sections .

4.1. Photosynthesis Studies

• Detects OEE3 expression levels under abiotic stress (e.g., cold, drought) to study PSII stability .

• Used to investigate OEE3’s role in repairing photodamaged PSII complexes .

4.2. Genetic Engineering

• Validates CRISPR/Cas9-mediated knockout or overexpression of Os07g0544800 in transgenic rice lines .

4.3. Comparative Plant Biology

• Compares OEE3 expression across species to understand evolutionary conservation of PSII components .

Technical Considerations

• Sample Preparation: Use chloroplast-enriched fractions for WB/IHC to minimize background .

• Controls: Include Os07g0544800 knockout rice mutants or pre-immune serum to confirm signal specificity .

• Dilution Range:

  • WB: 1:1,000–1:5,000

  • IHC: 1:100–1:500

Limitations and Future Directions

• Limitations: No data on reactivity in non-plant systems (e.g., algae) .

• Future Use: Potential for engineering stress-tolerant crops by modulating OEE3 expression .