PSMA7 Human
Description
Molecular Characterization of PSMA7
Definition: PSMA7 (Proteasome Subunit Alpha Type 7) is a 28 kDa protein encoded by the PSMA7 gene located at chromosome 20q13.33 . It forms part of the 20S core proteasome complex, which comprises two outer α-rings and two inner β-rings .
Core Proteasome Functions:
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Forms the α-ring "gate" regulating substrate entry into the proteolytic chamber .
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Facilitates ATP/ubiquitin-dependent degradation of misfolded proteins and regulatory factors (e.g., cyclins, transcription factors) .
Pathogen Interactions:
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Binds hepatitis B virus X protein (HBx) and modulates hepatitis C virus IRES activity, enhancing viral replication .
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Regulates hypoxia-inducible factor 1α (HIF-1α), influencing cellular responses to oxygen deprivation .
Immune Regulation:
Cancer Associations
Mechanisms:
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Activates NF-κB and MAPK pathways to promote proliferation .
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Interacts with PLK1 and HIF1A, driving cell cycle progression and angiogenesis .
Technical Insights
Recombinant Production:
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Expression: E. coli-derived PSMA7 (30 kDa with N-terminal His-tag) .
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Purity: >90% via chromatography; formulated in Tris-HCl/NaCl/glycerol .
Research Tools:
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Antibodies: Mouse monoclonal IgG1 (Clone 67817-1-Ig) validated for WB, IHC, and flow cytometry .
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Knockdown Models: shRNA-mediated PSMA7 silencing reduces anchorage-independent growth in RKO colorectal cells .
Therapeutic Potential
Targeted Inhibition:
Biomarker Utility:
Future Directions
Product Specs
Product Science Overview
PSMA7 is part of the alpha ring of the proteasome, contributing to the formation of heptameric alpha rings and the substrate entrance gate . The 20S proteasome complex, in association with different regulatory particles, forms the 26S proteasome. This complex is essential for the ATP-dependent degradation of ubiquitinated proteins, which is crucial for maintaining protein homeostasis by removing misfolded or damaged proteins .
PSMA7 is involved in various cellular processes, including:
- Protein Degradation: It mediates the degradation of intracellular proteins, both ubiquitin-dependent and ubiquitin-independent .
- Regulation of Transcription Factors: It inhibits the transactivation function of HIF-1A under both normoxic and hypoxic conditions .
- Immune Response: It plays a role in the generation of MHC class I-presented antigenic peptides .
- Cancer Progression: PSMA7 has been shown to promote proliferation and metastasis in gastric cancer through the MAPK signaling pathway .
Recombinant PSMA7 is used in research to study its structure, function, and role in various diseases. Understanding its mechanisms can lead to the development of new therapeutic strategies for diseases associated with proteasome dysfunction.
