PSMB4 Human

Proteasome Subunit Beta Type 4 Human Recombinant
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Cat. No.
BT6459
Source
Escherichia Coli.
Synonyms
Proteasome (prosome, macropain) subunit beta type 4, PROS26, HN3, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, Proteasome chain 3, 26 kDa prosomal protein, HsN3, PROS-26, hsBPROS26, EC 3.4.25.1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Gene Characteristics

  • Chromosomal Location: 1q21 .

  • Exons: 7 exons .

  • Orthologs: Conserved across species, including rat (Psmb4) and mouse (Psmb4) .

Protein Properties

  • Molecular Weight: 23 kDa (native), 26.6 kDa (recombinant with His-tag) .

  • Amino Acids: 219 (native), 240 (recombinant, residues 46–264) .

  • Isoelectric Point (pI): 5.47 .

  • Domains: Peptidase T1B family domain .

FeatureDetailsSource
Gene SymbolPSMB4
Protein FunctionNon-catalytic subunit of 20S proteasome; regulates ubiquitin-dependent degradation
Recombinant FormProduced in E. coli with N-terminal His-tag; purity >90%

Functional Roles

PSMB4 is integral to proteasome assembly and activity:

  • Core Proteasome Function: Forms the β-ring of the 20S proteasome, enabling cleavage of peptides via threonine-dependent catalysis .

  • Immunoproteasome Role: Processes antigens for MHC class I presentation, linking it to adaptive immunity .

  • Cellular Regulation:

    • Modulates NF-κB signaling to influence cell proliferation and apoptosis .

    • Interacts with SMAD1 to degrade transcriptional repressors like SNIP1 .

Cancer

  • Breast Cancer:

    • Overexpression correlates with tumor grade, size, and poor survival .

    • Silencing PSMB4 via siRNA reduces proliferation by inducing G1-phase arrest and downregulating cyclin D1 and PCNA .

  • Glioblastoma (GBM):

    • High PSMB4 expression associates with shorter survival. Knockdown inhibits migration and invasion via MMP-2/9 suppression .

  • Multiple Myeloma (MM):

    • Upregulated in patients with 1q21 amplification. PSMB4 inhibition synergizes with proteasome inhibitors (e.g., bortezomib) to enhance apoptosis .

Neuropsychiatric Disorders

  • Depression:

    • Plasma proteome-wide analysis identifies PSMB4 as a causal risk factor. MR analysis supports its role in depression etiology .

    • FDA-approved proteasome inhibitors (e.g., carfilzomib) are proposed for repurposing .

Biomarker Potential

  • Prognostic marker in breast cancer (HR = 2.1, P = 0.001) and GBM .

  • Correlates with 1q21 copy number in MM, independent of disease stage .

Therapeutic Targeting

  • Preclinical Evidence:

    • siRNA-mediated PSMB4 knockdown reduces tumor growth in xenograft models .

    • PSMB4 suppression sensitizes cancer cells to proteasome inhibitors .

  • Drug Candidates:

    • Bortezomib and carfilzomib directly target proteasome activity .

DiseaseKey FindingReference
Breast CancerPSMB4 siRNA reduces colony formation by 60–70%
GlioblastomaPSMB4 IHC score correlates with Ki-67 (r = 0.47)
DepressionMR analysis OR = 1.18 (P = 4.2 × 10⁻⁴)

Recombinant PSMB4 in Research

Recombinant human PSMB4 (e.g., RPPB2207) is widely used to study proteasome dynamics :

  • Applications: Protein interaction assays, drug screening, immunoproteasome studies.

  • Storage: Stable at -20°C in 30% glycerol .

Future Directions

  • Mechanistic Studies: Elucidate PSMB4’s role in NF-κB and p53 pathways.

  • Clinical Trials: Validate PSMB4-targeted therapies in MM and GBM.

  • Cross-Disciplinary Research: Explore PSMB4’s dual role in oncology and neuropsychiatry .

Product Specs

Introduction
PSMB4, a member of the peptidase T1B family, is a beta subunit of the 20S Proteasome. The 20S Proteasome chamber comprises structural alpha subunits and primarily catalytic beta subunits, arranged in two external rings of seven alpha subunits each and two internal rings of seven beta subunits each. PSMB4 is abundantly distributed throughout eukaryotic cells and participates in the ATP/ubiquitin-dependent degradation of peptides via a non-lysosomal pathway.
Description
Recombinant Human PSMB4, produced in E. coli, is a single, non-glycosylated polypeptide chain encompassing amino acids 46-264 (240 a.a.) and possessing a molecular weight of 26.6 kDa. This protein is expressed with an N-terminal 21 amino acid His-tag and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The PSMB4 protein solution (0.5 mg/ml) is supplied in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Proteasome (prosome, macropain) subunit beta type 4, PROS26, HN3, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, Proteasome chain 3, 26 kDa prosomal protein, HsN3, PROS-26, hsBPROS26, EC 3.4.25.1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTQNPMVTGT SVLGVKFEGG VVIAADMLGS YGSLARFRNI SRIMRVNNST MLGASGDYAD FQYLKQVLGQ MVIDEELLGD GHSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV LSQTEARDLV ERCMRVLYYR DARSYNRFQI ATVTEKGVEI EGPLSTETNW DIAHMISGFE

Product Science Overview

Introduction

Proteasome Subunit Beta Type 4 (PSMB4) is a crucial component of the proteasome complex, which plays a vital role in the degradation of intracellular proteins. This article delves into the structure, function, and significance of PSMB4, particularly focusing on its human recombinant form.

Structure and Composition

The proteasome is a multicatalytic proteinase complex with a highly ordered 20S core structure. This core is composed of four rings of 28 non-identical subunits: two rings consist of seven alpha subunits, and two rings consist of seven beta subunits . PSMB4 is one of these beta subunits, specifically known as the 20S core beta subunit .

The human PSMB4 protein is composed of 219 amino acids and has a molecular weight of approximately 23 kDa . It is encoded by the PSMB4 gene located on chromosome 1q21 .

Function

PSMB4 is a non-catalytic component of the 20S core proteasome complex . The proteasome complex is responsible for the proteolytic degradation of most intracellular proteins, which is essential for maintaining cellular homeostasis . This degradation process is ATP/ubiquitin-dependent and occurs in a non-lysosomal pathway .

The proteasome complex, including PSMB4, plays a critical role in various cellular processes such as:

  • Protein Quality Control: Degrading damaged or misfolded proteins to prevent their accumulation.
  • Regulation of Biological Processes: Degrading key regulatory proteins to control various cellular functions.
  • Immune Response: Processing class I MHC peptides through the immunoproteasome .
Significance of Human Recombinant PSMB4

Recombinant PSMB4 is produced using recombinant DNA technology, which allows for the expression of the human PSMB4 protein in various host systems. This recombinant form is essential for research and therapeutic applications, including:

  • Structural Studies: Understanding the detailed structure of PSMB4 and its interactions within the proteasome complex.
  • Functional Assays: Investigating the specific roles and mechanisms of PSMB4 in proteasome activity.
  • Drug Development: Screening for potential inhibitors or modulators of proteasome activity for therapeutic purposes.
Clinical Relevance

Mutations or dysregulation of PSMB4 have been associated with various diseases, including Proteasome-Associated Autoinflammatory Syndromes (PRAAS) and certain cancers . Understanding the function and regulation of PSMB4 is crucial for developing targeted therapies for these conditions.

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