bbs-9 Antibody

BBS-9 Antibody Overview

The BBS-9 antibody is a polyclonal rabbit IgG that recognizes specific epitopes of the BBS9 protein, which plays a structural and functional role in the BBSome—an octameric complex essential for trafficking membrane proteins to primary cilia . Key features include:

Key Applications

• Western Blot: Detects BBS9 at ~60–99 kDa across tissues (e.g., human heart, mouse testis) .

• Immunofluorescence: Localizes BBS9 to primary cilia in hTERT-RPE1 cells .

• Immunohistochemistry: Strong cytoplasmic staining in Purkinje cells (human cerebellum) .

• Functional Studies: Used to investigate BBS9’s role in osteogenic differentiation and ciliogenesis .

Recommended Dilutions

Disease Associations

• Bardet-Biedl Syndrome (BBS): Pathogenic mutations (e.g., L518P, N524Δ in BBS1) perturb BBS9 interactions, impairing ciliary trafficking .

• Craniosynostosis: Upregulated BBS9 splice variants disrupt osteogenic differentiation in calvarial sutures .

• Ciliopathies: Knockdown of BBS9 in zebrafish causes hydrocephalus and shortened cilia, mimicking human BBS phenotypes .

Functional Studies

• Cargo Transport: BBS9 facilitates Rab8-GTP localization to cilia, enabling vesicle docking .

• Protein Stability: The G182A mutation reduces BBS9 stability, accelerating degradation .

Critical Considerations

• Sodium Azide Warning: Some formulations contain sodium azide, requiring careful handling .

• Validation: Optimal dilutions vary by cell/tissue type; empirical testing is recommended .

• Species Cross-Reactivity: Predicted reactivity with cow (100%), dog (100%), and pig (100%) based on sequence homology .