PUF3 Antibody
Description
Overview of Puf3 Protein
Puf3 is a conserved RNA-binding protein belonging to the PUF (Pumilio and FBF) family. It regulates mRNA stability, translation, and localization by binding to specific motifs in the 3′ untranslated regions (3′UTRs) of target mRNAs .
RNA Targets
Puf3 binds mRNAs with a consensus motif (CNUGUAHAUA) and regulates transcripts involved in:
Protein Partners
Phosphorylation
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Hrr25-mediated phosphorylation reduces Puf3’s ability to degrade target mRNAs, enhancing mitochondrial protein synthesis .
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Metabolic state: Puf3 becomes hyperphosphorylated in respiratory conditions, stabilizing target mRNAs .
Phenotypic Effects of Puf3 Loss
Research Applications of Puf3 Antibody (Hypothetical)
While no studies in the provided sources utilized a PUF3-specific antibody, such a tool could enable:
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Localization studies: Tracking Puf3’s cytosolic or mitochondrial association via immunofluorescence.
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Co-immunoprecipitation: Validating interactions with Pbp1, Hrr25, or RNA targets.
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Post-translational modification analysis: Detecting phosphorylation states under varying metabolic conditions.
Key Unanswered Questions
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How does Puf3 coordinate nuclear and mitochondrial mRNA regulation?
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What structural features enable its dual roles in mRNA decay and translation?
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Are Puf3 homologs in pathogenic fungi (e.g., Candida) viable drug targets?
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Comprehensive multi-omics data indicate that PUF3 targets multiple classes of proteins, displaying coherent post-transcriptional regulatory properties. These findings suggest that PUF3 plays a broad but nuanced role in fine-tuning gene expression. PMID: 26493364
- Research findings indicate a likely role of PUF3 in controlling translation under oxidative stress conditions. This links its role in repression of protein synthesis to mitochondrially active genes. PMID: 24163252
- Data demonstrate that PUF3 exerts a general repressive effect on mitochondrial OXPHOS abundance, translation, and respiration. This effect does not involve changes in overall mitochondrial biogenesis and is largely independent of TORC1-mitochondrial signaling. PMID: 21655263
- While the PUF3 regulon is conserved among all studied fungi, the dedicated regulation of mitochondrial biogenesis by PUF3 emerged only in the Saccharomycotina clade. PMID: 20661438
- The PAB1-mRNP structure is crucial for PUF3 function. Moreover, multiple components of the CCR4-NOT deadenylase complex, but not PAN2, interacted with PUF3. PMID: 20435044
- PUF3 is a mitochondrial protein that contributes to the biogenesis and motility of the organelle. PMID: 17210948
- The activity of mRNA-binding proteins like PUF3 may provide the necessary conditions for fine spatiotemporal control of mitochondrial protein import and mitochondrial protein complex assembly. PMID: 18523582
KEGG: sce:YLL013C
STRING: 4932.YLL013C
