At5g51140 Antibody

Target Protein: At5g51140

The At5g51140 gene encodes a protein of uncharacterized function in Arabidopsis thaliana. While its exact role remains under investigation, homology studies suggest potential involvement in:

• Cellular localization: Predicted membrane association based on transmembrane domain analysis.

• Post-translational modifications: Presence of phosphorylation and glycosylation sites inferred from sequence motifs .

Research Applications

The At5g51140 antibody is primarily utilized for:

• Protein expression profiling: Tracking tissue-specific or stress-induced expression patterns in Arabidopsis.

• Subcellular localization studies: Immunohistochemical staining to determine cellular compartmentalization.

• Interaction studies: Co-immunoprecipitation (Co-IP) to identify binding partners in signaling pathways .

Example Workflow for Western Blot:

1. Sample Preparation: Extract proteins from Arabidopsis tissues under denaturing conditions.

2. Electrophoresis: Separate proteins via SDS-PAGE.

3. Transfer: Blot proteins onto a nitrocellulose membrane.

4. Incubation: Treat with At5g51140 antibody (1:1,000 dilution).

5. Detection: Use HRP-conjugated secondary antibodies and chemiluminescent substrates .

Gaps in Current Knowledge

Despite its commercial availability, peer-reviewed studies directly using the At5g51140 antibody are scarce. This highlights opportunities for:

• Functional characterization: Linking the protein to specific metabolic or developmental pathways.

• Comparative studies: Exploring orthologs in crop species for agricultural biotechnology applications .

Broader Context in Antibody-Based Plant Research

Monoclonal and polyclonal antibodies like At5g51140 are pivotal in plant sciences for:

• CRISPR-edited line validation: Confirming knockout/knock-in of target genes.

• Stress response studies: Quantifying defense-related proteins under abiotic/biotic stress .