pxn-1 Antibody

Role in Cancer and Metastasis

PXN promotes tumor migration and invasion by serving as a scaffold for integrin-mediated signaling. Phosphorylation at Y31/Y118 by PTK6 activates RAC1 via CRK, enhancing metastatic potential . Increased phospho-Y88 PXN correlates with colon cancer progression .

Mechanotransduction and Phosphorylation

• ULK1/2 kinases phosphorylate PXN at S32/S119 in response to mechanical stress, inhibiting actin polymerization and focal adhesion (FA) assembly .

• PXN−/− cells exhibit defective FA formation, rescued by wild-type PXN but hyperactivated by non-phosphorylatable mutants (e.g., S32A/S119A) .

Developmental Roles

In C. elegans, PXN-1 (a peroxidasin homolog) regulates neuronal development and heat-stress survival. PXN-1::GFP localizes to extracellular matrices, influencing axon guidance and muscle integrity .

Western Blot (WB)

• Sample preparation: Use RIPA buffer for lysates (e.g., HeLa cells).

• Dilution: 1:500–1:2000 for most antibodies .

• Band size: 64.5–68 kDa (varies by isoform and phosphorylation state) .

Immunohistochemistry (IHC)

• Antigen retrieval: EDTA buffer (pH 8.0) for paraffin-embedded tissues .

• Blocking: 10% goat serum reduces background .

Immunofluorescence (IF)

• PXN localizes to focal adhesions and actin-rich regions. Use DyLight®488 or DAPI for co-staining .

Emerging Insights from Mechanotransduction Studies