pyr-1 Antibody

Target Protein: PYR1

PYR1 is a member of the PYR/PYL/RCAR family of ABA receptors in Arabidopsis thaliana. It regulates ABA-dependent signaling by inhibiting protein phosphatase 2Cs (PP2Cs), thereby activating SnRK2 kinases to mediate stress responses . Structural studies reveal PYR1 binds ABA within a hydrophobic cavity, triggering conformational changes that enable PP2C interaction .

Key Functional Domains:

• ABA-binding pocket: Mediates hormone perception via residues like Lys59, Pro88, and Arg116 .

• Dimerization interface: Facilitates homodimer formation, critical for ABA sequestration and signaling .

Development and Validation of PYR1 Antibody

PYR1 antibodies are typically raised against synthetic peptides derived from the Arabidopsis PYR1 sequence. For example:

• Immunogen: KLH-conjugated peptide from Arabidopsis PYR1 (UniProt: O49686) .

• Host Species: Rabbit (polyclonal) or goat (polyclonal) .

• Applications: Western blot (WB), ELISA, immunoprecipitation (IP) .

Validation Data:

• Western Blot: Detects PYR1 at ~21 kDa in Arabidopsis protein extracts .

• Specificity: No cross-reactivity with other PYL family members confirmed via mutant analysis (pyr1 mutants show no signal) .

Research Applications and Findings

PYR1 antibody has been instrumental in elucidating ABA signaling mechanisms:

Table 1: Key Studies Using PYR1 Antibody

Recombinant Proteins:

• PYR1 (AA 1-191): His-tagged, >90% purity, expressed in yeast .

Technical Considerations

• Storage: Lyophilized antibodies stable at -20°C; avoid freeze-thaw cycles .

• Controls: Use pyr1 mutant plant extracts to confirm specificity .

• Limitations: Limited cross-reactivity outside Brassicaceae .