RAB26 Antibody, FITC conjugated

Definition and Core Characteristics

RAB26 Antibody, FITC conjugated is a fluorescently labeled immunological reagent designed for detecting RAB26, a small GTPase involved in intracellular vesicle trafficking and membrane dynamics . The fluorescein isothiocyanate (FITC) conjugation enables visualization of RAB26 localization and expression in assays such as immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry .

Key Features:

• Target: RAB26 (Ras-related protein Rab-26), a regulator of secretory granule transport and autophagy .

• Conjugate: FITC (excitation/emission: 495/519 nm), enabling fluorescence-based detection.

• Host Species: Rabbit (polyclonal) .

• Applications: Western blot (WB), ELISA, IF, IHC, immunoprecipitation (IP) .

Mechanistic Role of RAB26 in Cellular Processes

RAB26 regulates:

• Vesicle Trafficking: Mediates transport of α2-adrenergic receptors (ADRA2A/B) to the plasma membrane .

• Autophagy: Promotes autophagosome formation and degradation of phosphorylated Src kinase in breast cancer, suppressing metastasis .

• Secretory Granule Maturation: Critical for pepsinogen secretion in gastric cells .

Cancer Biology

• Breast Cancer:

  • Overexpression of RAB26 inhibits migration and invasion by degrading active Src via autophagy .

  • FITC-conjugated antibodies enable tracking of RAB26’s subcellular redistribution during autophagy (e.g., colocalization with LC3-positive autophagosomes) .

Immunology

• Acute Lung Injury (ALI):

  • Rab26 deficiency exacerbates inflammation by impairing M2 macrophage polarization. FITC labeling aids in studying Rab26-EPOR interactions in macrophages .

Neuroscience

• Synaptic Vesicle Dynamics:

  • Rab26 directs synaptic vesicles to autophagosomal degradation pathways. FITC conjugates facilitate live-cell imaging of vesicle trafficking in neurons .

Functional Insights

• Autophagic Degradation: Rab26 recruits ATG16L1 to mediate autophagic clearance of phosphorylated Src, reducing breast cancer invasiveness .

• Inflammatory Regulation: Rab26 knockdown amplifies pro-inflammatory cytokine release (TNF-α, IL-6) in macrophages during ALI .

Technical Validation

• Western Blot: Detects RAB26 at ~27 kDa in human cell lysates .

• Immunofluorescence: Localizes RAB26 to autophagosomes (co-staining with LC3) .

Comparison of RAB26 Antibodies

Critical Considerations

• Specificity: Validate using knockout controls due to potential cross-reactivity with other RAB GTPases.

• Storage: Prolonged exposure to light degrades FITC fluorescence; store in dark .

• Experimental Design: Pair with autophagy inhibitors (e.g., chloroquine) to study Rab26’s role in degradation pathways .