rab-6.2 Antibody

Introduction to RAB-and Its Antibody

RAB-6.2 is a Caenorhabditis elegans small GTPase orthologous to mammalian Rab6 proteins, which regulate retrograde trafficking between endosomes, Golgi, and the endoplasmic reticulum (ER) . Antibodies targeting Rab6 family proteins (including RAB-6.2 homologs) are critical tools for studying intracellular trafficking, synaptic receptor recycling, and cellular homeostasis. While no antibody exclusively targeting C. elegans RAB-6.2 has been explicitly described in the provided sources, commercially available antibodies against mammalian Rab6 isoforms (e.g., Rab6A, Rab6D) demonstrate cross-reactivity with RAB-6.2 in functional assays due to evolutionary conservation .

Retrograde Trafficking in C. elegans

• RAB-6.2 Function: Facilitates retrograde transport of AMPA-type glutamate receptors (GLR-1) and Wntless/MIG-14 in neurons and intestinal cells .

• Antibody Utility: While direct C. elegans RAB-6.2 antibodies are not listed, mammalian Rab6 antibodies (e.g., ab271094) are used in cross-species studies due to functional conservation. For example, mouse Rab6A rescues C. elegans rab-6.2 mutants, confirming homology .

Synaptic Receptor Recycling

• Mechanism: RAB-6.2 interacts with LIN-10 and the retromer complex to recycle GLR-1 receptors from endosomes to the trans-Golgi network (TGN), maintaining synaptic strength .

• Validation: Antibodies like #9625 enable detection of Rab6 in neuronal lysates, supporting studies on receptor trafficking .

Axon Regeneration Inhibition

• RAB-6.2 maintains APL-1 (APP homolog) protein levels in GABAergic motor neurons by diverting it from lysosomal degradation. Loss of rab-6.2 increases axon regeneration, suggesting its role in suppressing regenerative pathways .

• Key Interaction: RAB-6.2 depletion reduces APL-1-GFP fluorescence by 40–60% in C. elegans neurons, as quantified via CRISPR-tagged APL-1ΔC-GFP mutants .

Cuticle Integrity and Epidermal Function

• RAB-6.2 ensures epidermal integrity by regulating exocyst-mediated secretion. rab-6.2 mutants exhibit cuticle permeability defects, rescued by mammalian Rab6A expression .

Western Blot Performance

Immunoprecipitation (IP)

• ab271094: Efficiently immunoprecipitates Rab6A from A549 cell lysates, with no cross-reactivity in IgG controls .

• 10187-2-AP: Validated in IP assays using mouse brain lysates .