RAB9A Antibody
Description
Introduction
The RAB9A antibody is a critical research tool for studying the RAB9A protein, a member of the Rab GTPase family. RAB9A regulates vesicular trafficking, particularly the transport of mannose-6-phosphate receptors from late endosomes to the trans-Golgi network (TGN) . Its role in cancer progression, viral entry, and lysosomal biogenesis has made it a focal point for biomedical investigations. This review synthesizes data on RAB9A antibody applications, validation, and research findings from diverse sources.
Antibody Validation
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KD/KO Validation: Proteintech’s 11420-1-AP antibody has been validated in knockdown/knockout (KD/KO) models .
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Cross-reactivity: Species-specific reactivity includes human, mouse, rat, monkey, and hamster .
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Molecular Weight: All antibodies target the 23 kDa RAB9A protein .
Western Blotting (WB)
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Optimal Dilutions:
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Immunofluorescence (IF)
Oncogenic Role in Cancer
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Liver Cancer: RAB9A promotes proliferation, invasion, and survival via AKT/mTOR signaling . Antibodies (e.g., 11420-1-AP) confirmed downregulation of pro-apoptotic proteins (Bax, cleaved caspase-3) and upregulation of anti-apoptotic Bcl2 .
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Melanoma: Knockdown reduces tumor growth and induces apoptosis .
Viral Entry
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HPV Infection: Rab9a antibodies demonstrated its requirement for viral trafficking from endosomes to Golgi .
Therapeutic Implications
Product Specs
Target Background
- RAB9 competes with the catalytic subunit PPP2CA for binding to PPP2R1A. This competitive interaction plays a significant role in regulating PP2A catalytic activity. PMID: 27611305
- Protein kinase C modulates the transfer of alpha1B-adrenergic receptors to late endosomes, and RAB9 regulates this process, participating in the downregulation of G protein-mediated signaling. PMID: 28082304
- RAB9A and its co-regulatory GTPases control STX13-mediated cargo delivery to maturing melanosomes. PMID: 26527546
- RAB9-complexed TIP47 is essential for the proper release of hepatitis C viral particles. PMID: 24480419
- RAB9A and RAB7B mediate the transit of the L2 capsid protein and the pseudogenome of human papillomavirus 16 from the late endosome to the Golgi complex. PMID: 23345514
- RAB9A and RAB23 GTPases play crucial roles in the autophagy of Group A Streptococcus. PMID: 22452336
- These data show that RUTBC2 can act as a Rab36 GAP in cells, suggesting that RUTBC2 links RAB9A function to RAB36 function within the endosomal system. PMID: 22637480
- RUTBC1 is a Tre2/Bub2/Cdc16 domain-containing protein that binds to RAB9A-GTP both in vitro and in cultured cells, but does not function as a GTPase-activating protein for RAB9A. PMID: 21808068
- Data indicate that recombinant HPS1-HPS4 produced in insect cells can be efficiently isolated as a 1:1 heterodimer, and might function as a RAB9 effector in the biogenesis of lysosome-related organelles. PMID: 20048159
- Data show that ectopic expression of human telomerase reverse transcriptase (hTeRT) in human cells leads to an upregulation of the small GTPase RAB9 and its effector p40. PMID: 12576506
- The overall structure reveals a characteristic nucleotide binding fold consisting of a six-stranded beta-sheet surrounded by five alpha-helices with a tightly bound GDP molecule in the active site. PMID: 15263003
- Cholesterol contributes directly to the sequestration of RAB9 on Niemann-Pick type C cell membranes, which in turn disrupts mannose 6-phosphate receptor trafficking. PMID: 16644737
- Binding of the effector protein TIP47 is crucial for RAB9 localization. PMID: 16769818
- RAB9 interacts with the intermediate filament vimentin. This interaction is altered by lipid accumulation in late endosomes, resulting in inhibition of protein kinase C and hypophosphorylation of vimentin, leading to late endosome dysfunction. PMID: 18681838
- VV p37 protein associates with host TIP47, RAB9, and CI-MPR. PMID: 19400954
- Data indicate that human copper transporter 1 cleavage occurs in a late endosomal, RAB9-positive compartment prior to delivery of the transporter to the plasma membrane. PMID: 19684018
