Rabbit anti-Porcine IgG Antibody;HRP conjugated

Applications and Recommended Protocols

This antibody is widely used in enzyme-linked immunosorbent assays (ELISA), western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry (ICC) .

Optimized working dilutions:

Example protocol (Western blot):

1. Separate proteins via SDS-PAGE under reducing conditions.

2. Transfer to PVDF membrane and block with 5% non-fat milk.

3. Incubate with primary antibody (e.g., 1:1,000 dilution for 1 hour).

4. Apply Rabbit anti-Porcine IgG-HRP (1:5,000 dilution, 30 minutes) .

5. Detect using chemiluminescent substrates (e.g., TMB or ECL) .

Research Findings and Validation

• Western blot specificity: Demonstrated in detecting rabbit anti-HSP60 antibodies at 1:1,000 dilution, showing clear bands for HSP60 (60 kDa) without non-specific binding .

• IHC specificity: Successfully localized FoxP3 in human tonsil sections with minimal background when used at 1:20 dilution .

• F(ab')₂ specificity: Antibodies targeting porcine IgG F(ab')₂ fragments avoid Fc receptor-mediated false positives in flow cytometry and IHC .

Critical validations:

• Immunoelectrophoresis confirmed single precipitin arcs against swine IgG F(ab')₂ .

• No reactivity with non-target species (e.g., human, mouse) in ELISA .