Rabbit anti-Sheep IgG Antibody;HRP conjugated
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In Stock 
Description
Key Applications
This antibody is validated for multiple immunoassays, with performance varying by manufacturer-specific formulations:
Primary Uses
-
Western Blot (WB): Detects sheep IgG at dilutions of 1:2,000–1:20,000, depending on substrate sensitivity .
-
Immunohistochemistry (IHC): Compatible with frozen sections and cytochemical staining .
Secondary Uses
Research Findings and Validation
The antibody has been cited in peer-reviewed studies for target detection and signal amplification:
Recommended Dilutions
| Application | Dilution Range | Substrate Type |
|---|---|---|
| Western Blot | 1:2,000–1:10,000 | ECL (chemiluminescent) |
| ELISA | 1:100–1:20,000 | TMB (chromogenic) |
Limitations and Considerations
Product Specs
Buffer
**Preservative:** 0.03% Proclin 300
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Our standard lead time for dispatching this product is 1-3 working days following receipt of your order. Delivery times may vary depending on the purchasing method and location. Please consult your local distributor for specific delivery details.
Customer Reviews
,
B.A
By Anonymous
★★★★★
Applications : Immunohistochemical stain
Sample type: tissue
Sample dilution: 1:500
Review: For the detection of cTnI, the sections were incubated with the sheep polyclonal anti-cTnI antibody and then HRP-conjugated rabbit antisheep IgG secondary antibody.
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