Rad1 Antibody

DNA Damage Response and Repair

• RAD1 forms the 9-1-1 complex with Rad9 and Hus1, which stabilizes DNA repair enzymes like FEN1 and DNA ligase I .

• The complex is recruited to double-strand breaks (DSBs) during S phase, facilitating homologous recombination .

• Sumoylation at lysine 32 (K32) regulates RAD1’s dissociation from DNA post-repair, enhancing efficiency in high-damage conditions .

Cancer and Genomic Stability

• Knockout studies in mice show that RAD1 deletion increases susceptibility to skin tumors (e.g., DMBA-induced papillomas) .

• Overexpression of Rad9 (a binding partner) correlates with poor prognosis in ovarian and prostate cancers .

• Jab1/CSN5-mediated degradation of the 9-1-1 complex suppresses checkpoint signaling, accelerating genomic instability .

Table 1: Experimental Models and Outcomes

Applications in Research

• DNA Repair Studies: Rad1 antibodies identify RAD1 localization at DSBs via IF/ICC .

• Cancer Biomarker Screening: Used to correlate RAD1 expression levels with tumor progression .

• Mechanistic Studies: Detect sumoylation or phosphorylation states in response to UV/CPT treatment .

Technical Considerations

• Storage: Stable at -20°C in PBS with 0.02% sodium azide .

• Controls: Recommended cell lines include HeLa, A431, and 293T for WB validation .

• Limitations: Cross-reactivity with RAD1 isoforms (3 splice variants) may require isoform-specific validation .