RAD9B Antibody, FITC conjugated

RAD9B Antibody, FITC Conjugated: Definition and Applications

The RAD9B antibody, FITC conjugated, is a polyclonal rabbit-derived antibody covalently linked to fluorescein isothiocyanate (FITC), a fluorescent dye emitting green light. Its primary function is to bind specifically to the RAD9B protein, facilitating downstream applications such as:

• Immunohistochemistry (IHC): Detection of RAD9B in paraffin-embedded tissue sections .

• Flow Cytometry (FCM): Quantitation of RAD9B expression in live or fixed cells .

• Western Blotting (WB): Validation of RAD9B protein presence in lysates .

This conjugation enhances the antibody’s utility in fluorescence microscopy, enabling co-localization studies with other cellular markers.

Role in DNA Damage Response

RAD9B is a checkpoint protein involved in:

• DNA Replication Checkpoints: Ensuring genomic stability during replication .

• Intra-S Phase Damage Response: Repairing DNA lesions to prevent replication fork collapse .

In breast cancer studies, RAD9B overexpression correlates with reduced chemosensitivity to doxorubicin, suggesting its role in therapeutic resistance . FITC-conjugated antibodies could potentially identify RAD9B expression patterns in tumor biopsies, aiding in personalized treatment strategies.

Mechanistic Insights

• Checkpoint Activation: RAD9B interacts with CHK1, a kinase that regulates cell cycle arrest following DNA damage .

• Apoptosis Regulation: RAD9B deficiency increases apoptosis in B cells, indicating its role in survival signaling .

Practical Considerations for Use

• Optimization: Titration experiments are critical for optimal signal-to-noise ratios in IHC and FCM .

• Controls: Include isotype-matched IgG controls to confirm specificity .

• Cross-Reactivity: Verify reactivity with target species (e.g., human vs. mouse) .