rae-1 Antibody
Description
What is RAE-1 Antibody?
RAE-1 antibodies are monoclonal or polyclonal reagents designed to detect and study RAE-1 proteins. These antibodies are critical for understanding RAE-1's dual roles in immune activation and tumor biology. Key features include:
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Target Specificity: Recognizes RAE-1 isoforms (e.g., RD-41 clone targets RAE-1δ ).
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Applications: Flow cytometry, Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA .
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Species Reactivity: Primarily validated in mice and humans .
Key Applications of RAE-1 Antibodies
RAE-1 antibodies are utilized across diverse experimental contexts:
Immune Surveillance and Tumor Rejection
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RAE-1 overexpression on MHC class I⁺ tumors (e.g., RMA lymphoma) triggers NK cell-mediated rejection in vivo, bypassing the "missing self" hypothesis .
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MDSCs expressing RAE-1 activate NK cells via NKG2D, enhancing IFN-γ production and antitumor responses .
Cancer Progression and Metastasis
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Breast Cancer: RAE-1 promotes epithelial-mesenchymal transition (EMT) by upregulating ZEB1, driving metastasis .
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Colorectal Cancer: RAE-1 overexpression stabilizes spindle bipolarity, inhibits apoptosis, and induces chemoresistance .
Mitotic Regulation
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RAE-1 ensures proper spindle formation during mitosis; its dysregulation causes chromosome instability and tumorigenesis .
Critical Considerations
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Isoform Cross-Reactivity: Some antibodies (e.g., "pan-RAE-1") detect multiple isoforms, while others (e.g., RD-41) are isoform-specific .
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Experimental Optimization: Titration is essential for assays like IHC, where antigen retrieval methods vary (e.g., TE buffer pH 9.0 vs. citrate buffer pH 6.0) .
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Species Limitations: Most antibodies are validated for murine or human systems; cross-reactivity with other species is untested .
Future Directions
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
