ranbp9 Antibody
Description
Definition and Core Applications of RanBP9 Antibody
The RanBP9 antibody is designed to detect and study RanBP9, an evolutionarily conserved scaffold protein involved in diverse cellular processes . Key applications include:
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Western blot (WB): Detects RanBP9 at ~78 kDa (full-length) and proteolytic fragments (e.g., ~60 kDa) .
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Immunoprecipitation (IP): Identifies RanBP9 interaction partners, such as ATM kinase and Nucleolin .
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Immunohistochemistry (IHC): Localizes RanBP9 in tissue samples, revealing nuclear-cytoplasmic shuttling dynamics .
DNA Damage Response (DDR)
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ATM Phosphorylation: RanBP9 is phosphorylated by ATM at S181 and S603 following ionizing radiation (IR), facilitating its nuclear accumulation .
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DDR Regulation: Silencing RanBP9 delays activation of ATM, Chk2, γH2AX, and p53, impairing homologous recombination repair and increasing IR-induced senescence/apoptosis .
Table 1: Key DDR-Related Findings
| Parameter | Observation | Citation |
|---|---|---|
| Phosphorylation Sites | S181, S603 (ATM-dependent) | |
| γH2AX Dynamics | Reduced early (<4 h), elevated at 24 h | |
| HR Repair Efficiency | ↓ 50% in RanBP9-silenced cells |
Mitochondrial Apoptosis
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RanBP9 overexpression increases Bax/Bcl-2 ratio, cytochrome c release, and mitochondrial ROS, promoting apoptosis in neuronal cells .
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Co-localizes with transcription factor p73 in mitochondria, enhancing p73 stability and pro-apoptotic activity .
Neurodegeneration
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A 60 kDa RanBP9 fragment (N60) is elevated 6-fold in Alzheimer’s disease (AD) brains, correlating with amyloid-β peptide generation .
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Combined full-length and N60 RanBP9 levels are 75% higher in AD vs. controls .
Cancer
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RanBP9 deficiency reduces ATM acetylation, sensitizing lung cancer cells to genotoxic therapies .
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Tagged RanBP9 mouse models (V5-HA) reveal interactions with Nucleolin, a cancer therapeutic target .
Antibody Performance
Table 2: Anti-RanBP9 Antibody Specifications (ab205954)
| Parameter | Detail |
|---|---|
| Host Species | Rabbit |
| Applications | WB, IP |
| Predicted Band Size | 78 kDa |
| Optimal Lysate Loading | 50 µg (WB); 1 mg (IP) |
Enhanced Detection Methods
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RanBP9-TT Mice: CRISPR/Cas9-engineered mice with C-terminal V5-HA tags enable high-sensitivity detection (e.g., liver samples) .
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Tagged RanBP9 retains native interactions (e.g., with Nucleolin) and resolves antibody specificity issues .
Research Implications
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Research indicates that RanBP9 plays a significant role in retinal cell development during zebrafish embryogenesis. PMID: 28359144
