RANBP9 Antibody, Biotin conjugated
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Description
General Overview of RANBP9 Antibodies
RANBP9 (Ran-binding protein 9), also known as RanBPM, is a scaffolding protein involved in diverse cellular processes, including receptor internalization, signal transduction, and amyloid precursor protein (APP) processing . Antibodies targeting RANBP9 are critical tools for studying its role in neurodegenerative diseases, such as Alzheimer's disease, where it enhances β-secretase (BACE1) cleavage of APP .
A biotin-conjugated RANBP9 antibody would typically involve a primary antibody chemically linked to biotin, enabling detection via streptavidin-enzyme conjugates (e.g., HRP or alkaline phosphatase) in techniques like ELISA, Western blot (WB), or immunohistochemistry (IHC).
Table 1: Comparison of RANBP9 Antibodies
Key Research Findings Using RANBP9 Antibodies
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Role in APP Processing: Studies demonstrate that RANBP9 overexpression reduces cell surface APP by 52% and accelerates APP internalization, promoting β-secretase cleavage and amyloid-β (Aβ) generation .
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Domain Interactions: The SPRY-LisH domains of RANBP9 mediate interactions with APP, LRP, and BACE1, facilitating Aβ production .
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Validation Data:
Hypothetical Applications of a Biotin-Conjugated RANBP9 Antibody
If developed, a biotin-conjugated RANBP9 antibody might enable:
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Enhanced Signal Amplification: Streptavidin-biotin systems improve sensitivity in low-abundance protein detection.
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Multiplex Assays: Compatibility with avidin-coated plates or beads for high-throughput screening.
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Cross-Species Studies: Given RANBP9’s conservation, such an antibody could be tested in human, mouse, and rat models .
Limitations and Considerations
Product Specs
Buffer
Preservative: 0.03% ProClin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Orders are typically shipped within 1-3 business days. Delivery times may vary depending on the shipping method and destination. Please contact your local distributor for precise delivery estimates.
Synonyms
B cell antigen receptor Ig beta associated protein 1 antibody; BPM 90 antibody; BPM L antibody; BPM-L antibody; BPM90 antibody; BPML antibody; IBAP 1 antibody; Imp 9 antibody; Importin 9 antibody; Novel centrosomal protein RanBPM antibody; RAN binding protein 9 antibody; Ran binding protein centrosomal antibody; Ran Binding Protein in the Microtubule organizing center antibody; Ran binding protein M antibody; Ran BP9 antibody; Ran-binding protein 9 antibody; Ran-binding protein M antibody; RANB9_HUMAN antibody; RanBP 7 antibody; RANBP 9 antibody; RanBP7 antibody; RanBP9 antibody; RanBPM antibody
Target Names
RANBP9
Uniprot No.
Target Background
Function
RanBPM (Ran Binding Protein 9) is a multifunctional protein that likely acts as a scaffolding and adapter protein, linking membrane receptors to intracellular signaling pathways. It plays a role in cell spreading and actin cytoskeleton rearrangement. It's a core component of the CTLH E3 ubiquitin-protein ligase complex, mediating the ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. RanBPM may be involved in integrin signaling (e.g., ITGB2/LFA-1) and enhances HGF-MET signaling by recruiting Sos and activating the Ras pathway. It also modulates the transactivation activity of several receptors (AR, NR3C1), and stabilizes TP73α, increasing its proapoptotic activity. Furthermore, it inhibits the kinase activity of DYRK1A and DYRK1B, and inhibits FMR1 binding to RNA.
Gene References Into Functions
RanBPM's diverse roles are extensively documented in the literature. Key findings include:
- Comprehensive review of RanBPM interactors, its cellular functions, and therapeutic potential: PMID: 29723489
- Modulatory role in IFN-stimulated response elements, impacting IFN-lambda signaling through interaction with IFN-lambdaR1: PMID: 28547582
- Association between high RANBPM expression and cancer: PMID: 26919101
- Cooperative suppression of metastasis with RanBP9/TSSC3 complex via downregulation of the Src-dependent Akt pathway and induction of anoikis: PMID: 28032865
- Inhibitory role in lung cancer cell proliferation and invasion, associated with improved prognosis: PMID: 28739732
- Interaction with DDX-4, an RNA helicase involved in germ cell development: PMID: 27622290
- Negative regulation of BLT2 and IL8, attenuating invasiveness in aggressive breast cancer cells: PMID: 28027932
- Impaired DNA repair and increased sensitivity to genotoxic stress in the absence of RanBP9: PMID: 26943034
- Role of RanBPM loss in gastric cancer development, metastasis, and chemoresistance: PMID: 26977028
- Positive RanBP9 expression in bone tumor tissues and cell lines: PMID: 27049080
- Enhancement of Zta-dependent transcriptional activity by inhibiting Zta sumoylation: PMID: 25900136
- Lack of association between RANBP9 variants and schizophrenia risk: PMID: 25482375
- Relocation of APP intracellular domain to Tip60-enriched nuclear speckles by RanBP9, impacting AbetaPP signaling: PMID: 25024339
- RanBPM subcellular localization influenced by multiple factors impacting nucleocytoplasmic transport: PMID: 25659156
- Early synaptic deficits, impaired learning, and accelerated amyloid plaque accumulation with RanBP9 transgene overexpression: PMID: 24254706
- COPS5 as a RanBP9-binding protein increasing APP processing and Abeta generation: PMID: 23926111
- RanBPM as a negative regulator of BLT2 signaling, attenuating cell motility: PMID: 23928309
- Influence of RanBPM on TRAF6 ubiquitination and NF-kappaB signaling through interaction with TRAF6: PMID: 21805090
- Simultaneous inhibition of cell-adhesive processes and enhancement of Abeta generation by RanBP9 via endocytosis acceleration: PMID: 22223749
- Role of RanBPM in neocortical precursor progression through M-phase: PMID: 19790105
- Potential modulation of synaptic plasticity through RanBPM co-localization with p42IP4: PMID: 18298663
- RanBP9 and RanBP10 as signaling integrators regulating D(1) receptor signaling: PMID: 20395553
- Modulation of TrkB-mediated signaling and biological functions by RanBPM: PMID: 20403074
- Proapoptotic function in DNA damage-induced apoptosis through regulation of mitochondrial apoptotic pathway factors: PMID: 19996306
- Increased RanBP9-N60 in Alzheimer's disease brains: PMID: 19729516
- RanBPM as an enzymatic substrate for USP11, undergoing specific deubiquitination: PMID: 12084015
- Role in HGF-MET and Ras signal transduction pathways: PMID: 12147692
- Interaction with HIPK2 in the nucleus of mammalian cells: PMID: 12220523
- Selective modification of steroid receptor activity: PMID: 12361945
- Direct interaction with CDK11(p46) in vitro and in human cells: PMID: 14511641
- Potential role as a molecular scaffold coupling LFA-1 and other integrins with intracellular signaling pathways: PMID: 14722085
- Inhibition of p73alpha ubiquitination and prolongation of its half-life: PMID: 15558019
- Interaction with receptor tyrosine kinases Axl and Sky: PMID: 15964779
- Synergistic inhibition of MyoD-directed muscle reporter gene transcription with constitutively active Raf kinase: PMID: 16364241
- Potential regulation of NTPDase catalytic activity through association with CD39: PMID: 16478441
- Coactivator function for thyroid hormone receptors: PMID: 16595702
- Complex formation with ARMC8alpha, ARMC8beta, Muskelin, p48EMLP, and p44CTLH: PMID: 17467196
- Scaffolding protein role in regulating cellular function in the immune and nervous systems: PMID: 18040864
- Enhancement of Epstein-Barr virus Rta protein transactivation activity: PMID: 18455188
- Role of muskelin-RanBP9 complex in integrating cell morphology regulation and nucleocytoplasmic communication: PMID: 18710924
- Potential key regulatory role in Ca(v)3.1 channel-mediated signaling pathways: PMID: 18801335
- Enhancement of APP interactions with LRP and BACE1 and increased lipid raft association of APP upon RanBP9 overexpression: PMID: 19251705
Database Links
Protein Families
RANBP9/10 family
Subcellular Location
Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein.
Tissue Specificity
Ubiquitously expressed, with highest levels in testes, placenta, heart, and muscle, and lowest levels in lung. Within the brain, expressed predominantly by neurons in the gray matter of cortex, the granular layer of cerebellum and the Purkinje cells.
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