rsef-1 Antibody
Description
Validated Applications
| Application | Confirmed Detection | Recommended Dilution |
|---|---|---|
| Western Blot | LNCaP prostate cancer cell line | 1:1000-1:6000 |
| Immunohistochemistry | Human prostate/ovarian/pancreatic tumors, Mouse pancreas | 1:500-1:2000 |
| Immunofluorescence | A431 epidermal carcinoma cells | 1:200-1:800 |
The antibody requires antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) for optimal IHC performance in formalin-fixed paraffin-embedded tissues .
Target Biology
RASEF protein contains:
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RAS-association (RA) domain
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EF-hand calcium-binding motifs
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Coiled-coil regions
These structural features suggest roles in:
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Small GTPase signaling pathways
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Calcium-dependent cellular processes
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Protein-protein interaction networks
The observed molecular weight discrepancy (90 kDa vs calculated 83 kDa) may result from post-translational modifications or alternative splicing variants .
Quality Control
| Parameter | Specification |
|---|---|
| Batch Consistency | Protein A purified, lot-specific QC |
| Stability | 12 months at -20°C |
| Cross-reactivity | None reported against common human/mouse tissues |
Users should perform antibody titration for each experimental system and validate results against positive controls included in the product validation data gallery .
Research Applications
This antibody enables:
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Cancer biomarker studies (prostate, ovarian, pancreatic)
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Subcellular localization analysis
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Protein expression profiling across species
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Molecular interaction studies through co-immunoprecipitation
Product Specs
Composition: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
