RASSF2 Antibody, HRP conjugated
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Description
Overview of RASSF2 Antibody, HRP Conjugated
RASSF2 Antibody, HRP conjugated is a horseradish peroxidase (HRP)-linked antibody targeting the Ras association domain-containing protein 2 (RASSF2). This antibody enables high-sensitivity detection of RASSF2 in techniques such as Western blotting (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA) . RASSF2 itself is a scaffold protein involved in apoptosis, cell cycle regulation, and Ras-mediated signaling .
Antody Structure
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Host Species: Available in mouse monoclonal (IgG1κ) and rabbit polyclonal formats .
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Epitope: Targets specific regions of RASSF2, such as amino acids 201–300 (human) or internal domains .
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Conjugate: HRP enables chromogenic or chemiluminescent detection via enzymatic substrate conversion .
RASSF2 Protein Features
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Domains: Contains a Ras-associating (RA) domain and a SARAH domain critical for interactions with MST1/2 kinases .
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Function: Stabilizes MST2 kinase, promotes apoptosis, and acts as a tumor suppressor by regulating Ras signaling .
Applications in Research
The HRP-conjugated RASSF2 antibody is validated for multiple applications:
Interaction with MST1/2 Kinases
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RASSF2 forms a stable complex with MST2 via its SARAH domain, protecting MST2 from proteasomal degradation and enhancing its proapoptotic activity .
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Mutation of the SARAH domain (e.g., L290P) disrupts this interaction, confirming its structural necessity .
Role in Cancer
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RASSF2 is frequently epigenetically silenced in colorectal cancer via promoter hypermethylation .
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Overexpression of RASSF2 suppresses tumor growth by stabilizing MST2 and promoting apoptosis .
Novel Binding Partners
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship the products within 1-3 business days of receiving your order. Delivery times may vary depending on the mode of purchase or location. For specific delivery times, please consult your local distributors.
Synonyms
CENP 34 antibody; Centromere protein 34 antibody; Ras association (RalGDS/AF 6) domain family 2 antibody; Ras association (RalGDS/AF-6) domain family member 2 antibody; Ras association domain containing protein 2 antibody; Ras association domain-containing protein 2 antibody; RASF2_HUMAN antibody; RASFADIN antibody; RASSF2 antibody; RP4-599I11.1 antibody
Target Names
RASSF2
Uniprot No.
Target Background
Function
RASSF2 is a potential tumor suppressor that functions as a KRAS-specific effector protein. It may promote apoptosis and cell cycle arrest. RASSF2 stabilizes STK3/MST2 by preventing its degradation through the proteasome.
Gene References Into Functions
Gene References and Functions
- Studies have shown that the methylation levels of the RASSF2A promoter are significantly higher in endometrial adenocarcinoma (OEA group) and ovarian clear cell carcinoma (OCC group) compared to other groups. The expression level of RASSF2A mRNA was also lower in the OEA and OCC groups. PMID: 29504361
- Through bioinformatics methods, we confirmed that the RASSF2-PAR-4 axis is primarily responsible for the functions of miR-7 in CAFs. Overexpression of miR-7 in CAFs resulted in a decrease in RASSF2 expression, significantly reducing PAR-4 secretion from CAFs. This, in turn, enhanced the proliferation and migration of co-cultured cancer cells. PMID: 27901488
- The findings suggest that downregulation of RASSF2 and RASSF6 is a tumor-specific phenomenon. Their inactivation through CpG island methylation may play crucial roles in the development of gastric cardia cancer. PMID: 26456015
- The statistically most significant association, rs4813720, is correlated with RASSF2 expression and may play a significant role in the etiology of childhood ALL. PMID: 27861356
- RASSF2 could be a critical molecular marker for carcinogenesis, prognosis, and progression in gastric cancer. PMID: 26459248
- The RASSF2 gene exhibits differential methylation in luminal and non-luminal tumors and is a promising suppressor gene with clinical relevance in breast cancer. PMID: 26284587
- Our results suggest that RASSF2 may act as a tumor suppressor gene. PMID: 26482475
- We conclude that EPB41L3, RASSF2, and TSP-1 genes are implicated in the pathogenesis of diffuse gliomas. PMID: 25621889
- Promoter hypermethylation of RASSF2A is observed in cervical cancer, but not in normal cervical tissues. PMID: 24605823
- Epigenetic inactivation of RASSF2A through aberrant promoter methylation might play a significant role in the development of epithelial ovarian cancer. PMID: 24606436
- The findings suggest that both RASSF1A and RASSF2 are novel epigenetically inactivated tumor suppressor genes in Ewing sarcoma. Methylation of RASSF2 may have prognostic implications for ES patients. PMID: 23887284
- Our results suggest that RASSF2 potentially functions as a new tumor-suppressor gene inactivated through hypermethylation in cervical cancer. PMID: 23542458
- Promoter hypermethylation of RASSF2A is observed in pancreatic ductal adenocarcinoma, but not in normal pancreatic tissue. PMID: 21792082
- Hypermethylation of the RASSF2 gene is associated with advanced gastric cancer. PMID: 21409489
- Sp1-mediated RASSF2A gene transcription is activated by p300 through histone acetylation, and this activation plays a critical role in inducing late apoptosis. PMID: 20716062
- Results suggest that RASSF2 encodes a novel epigenetically inactivated candidate tumor suppressor gene in thyroid carcinogenesis. PMID: 20920251
- Aberrant methylation of the RASSF2A gene with the subsequent loss of RASSF2A expression plays a crucial role in the pathogenesis of hepatocellular carcinoma. PMID: 19513612
- Data show that RASSF2 forms a direct and endogenous complex with prostate apoptosis response protein 4 (PAR-4). This interaction is regulated by K-Ras and is essential for the full apoptotic effects of PAR-4. PMID: 20368356
- These findings indicate that MST1 is a major determinant of RASSF2 protein stability, and suggest that RASSF2 acts in a complex manner that extends beyond simple protein-protein association to play a significant role in MST1 regulation. PMID: 19962960
- RASSF2 is a new member of the RASSF1 family of Ras effectors/tumor suppressors that exhibits a specificity for interacting with K-Ras. PMID: 12732644
- In colonic neoplasms, epigenetic (RASSF2A) and genetic (K-ras) changes are mutually exclusive and provide alternative pathways for affecting Ras signaling. PMID: 15806169
- RASSF2 is a novel tumor suppressor gene that regulates Ras signaling and plays a pivotal role in the early stages of colorectal tumorigenesis. PMID: 16012945
- RASSF2 is silenced by hypermethylation near the transcription start site in gastric cancer. PMID: 16265349
- Inactivation of RASSF2A by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma. PMID: 17013896
- Hypermethylation of the RASSF2A promoter and K-ras/BRAF mutations are associated with microsatellite-stable colorectal cancers. PMID: 17013898
- Transport of Ras effector superfamily proteins into the nucleus/nucleolus may play a crucial role in modulating Ras-mediated cell proliferation during tumorigenesis. PMID: 17320110
- Aberrant methylation of the RASSF2 gene with the subsequent loss of RASSF2 expression plays a crucial role in the pathogenesis of lung cancers. PMID: 17549418
- A novel methylation marker for multiple malignancies; suppresses breast tumor cell growth in vitro and in vivo. PMID: 17891178
- Epigenetic silencing of tumor suppressor genes involved in the Ras/PI3K/AKT pathway plays a significant role in oral squamous cell carcinoma radioresistance. PMID: 19509163
- MST2 and RASSF2 form an active complex in vivo, where RASSF2 is maintained in a phosphorylated state and protects MST2 from degradation and turnover. PMID: 19525978
- Data provide evidence that MAPK/ERK-2 mediated phosphorylation regulates nucleo-cytoplasmic transport and cell growth arrest activity of RASSF2. PMID: 19555684
- Tumor-specific methylation of APC, MGMT, RASSF2A, and Wif-1 genes might be a valuable biomarker in plasma for the early detection of colorectal cancer. PMID: 19773381
Database Links
Subcellular Location
Nucleus. Cytoplasm. Chromosome, centromere, kinetochore. Note=Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.
Tissue Specificity
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
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