IL6 Rat, sf9
Description
Production Methodology
The baculovirus expression system in Sf9 cells offers advantages for IL6 Rat, sf9 synthesis:
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Gene Insertion: The rat IL6 coding sequence (excluding the signal peptide) is cloned into a baculovirus transfer vector.
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Transfection: The vector is co-transfected with linearized baculovirus DNA into Sf9 cells.
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Protein Secretion: Recombinant IL6 is secreted into the culture medium and purified via proprietary chromatography .
Key Advantages:
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High Yield: Sf9 cells produce milligram quantities per liter of culture .
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Native Modifications: Glycosylation mimics mammalian systems, enhancing bioactivity .
Inflammation and Immune Response
IL6 Rat, sf9 is pivotal in studying inflammatory pathways:
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Acute Phase Response: Induces transcription of hepatic proteins like fibrinogen and CRP .
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Trans-Signaling: Binds soluble IL-6Rα to activate gp130 on cells lacking membrane-bound IL-6Rα, expanding target cell range .
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Experimental Models: Used to study cystitis in rats, where IL6 overexpression correlates with bladder hyperreflexia .
Experimental Findings:
Source:
Neurobiology and Cancer
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Neuroprotection: Astrocyte-derived IL6 promotes neuronal differentiation in hippocampal progenitor cells .
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Oncology: IL6 signaling via gp130 drives cancer progression in models of leukemia and solid tumors .
Disease Associations
IL6 Rat, sf9 has been implicated in:
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Autoimmune Diseases: Rheumatoid arthritis, systemic juvenile idiopathic arthritis .
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Viral Infections: Elevated IL6 levels correlate with COVID-19 severity .
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Metabolic Disorders: Links to insulin resistance and obesity .
Production Challenges
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Optimization: Scale-up requires precise control of baculovirus titers and cell viability .
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Quality Control: Ensuring batch consistency in glycosylation and bioactivity remains critical .
Therapeutic Potential
Product Specs
Interleukin-6, a potent pro-inflammatory cytokine, is mainly produced by activated T cells and various other cells, such as endothelial cells and macrophages. IL-6 influences both B and T lymphocytes and has been found to play a role in host defense mechanisms, acute phase reactions, immune responses, and hematopoiesis.
IL6, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 196 amino acids (25-211 a.a.) with a molecular mass of 22.8kDa. Note: On SDS-PAGE, the molecular size will appear between 18-28 kDa.
This IL6 is expressed with a C-terminal 6 amino acid His tag and purified using proprietary chromatographic techniques.
The IL6 protein solution (0.5mg/ml) is supplied in a buffer containing 20% glycerol and Phosphate Buffered Saline (pH 7.4).
Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Purity is determined to be greater than 95.0% via SDS-PAGE analysis.
Biological activity is assessed using a cell proliferation assay with M-NFS-60 mouse B cells. The ED50 for this effect is found to be less than or equal to 0.1 ng/ml.
IL6, Ifnb2, ILg6.
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Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune regulation, inflammation, and hematopoiesis. It is primarily produced by activated T cells, endothelial cells, and macrophages. IL-6 is involved in various physiological processes, including host defense, acute phase reactions, and immune responses .
The production of recombinant IL-6 has been a significant advancement in biomedical research. Rat Recombinant IL-6 is produced using the baculovirus expression system in Sf9 cells, which are derived from the Spodoptera frugiperda insect cells . This system allows for efficient and high-yield production of recombinant proteins.
The recombinant IL-6 produced in Sf9 cells is a single, glycosylated polypeptide chain containing 196 amino acids and has a molecular mass of approximately 22.8 kDa . It is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques .
Rat Recombinant IL-6 has widespread applications in research and therapeutic development. It serves as a valuable tool for investigating the mechanisms underlying IL-6-mediated immune responses, inflammation, and hematopoiesis . Some of the key applications include:
- Immunology Research: IL-6 is used to study its role in immune regulation and the acute phase response.
- Disease Research: It helps in understanding the involvement of IL-6 in various diseases, including autoimmune disorders and cancers.
- Therapeutic Development: Recombinant IL-6 is used in the development of potential therapies targeting IL-6 pathways.
The IL-6 protein solution is formulated with 20% glycerol and Phosphate Buffered Saline (pH 7.4). For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the solution frozen at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
